Ayanoglou C M, Godeau G, Lesty C, Septier D, Goldberg M
Laboratoire Biologie et Biomatériaux du Milieu Buccal et Osseux, Faculté de Chlrurgie Dentaire, Université Paris V, Montrouge, France.
J Oral Pathol Med. 1997 Mar;26(3):129-34. doi: 10.1111/j.1600-0714.1997.tb00035.x.
Cyclosporin A (CsA), a widely used immunosuppressive drug, induces gingival overgrowth and modifications of bone remodelling. The scope of this study was to investigate the possible effect of CsA on dentin. Thirty mg/kg/day of CsA were administered orally to male Sprague-Dawley rats for nineteen weeks. The same number of control rats received oil-based vehicle solution. Rats were anesthetized, and tissues were fixed by an intracardiac perfusion of fixative solution. Mandibles were dissected, demineralized, and processed for Epon embedding. Semi-thin sections of the first molars revealed alterations at the secondary dentin-pulp interface in four out of six experimental animals. The changes consisted of the formation of: 1) osteodentin spurs, in which the volume and interface with the secondary dentin varied from about 25,000 to 75,000 microns 3 and from 1400 to 3530 microns 2, respectively; 2) abnormally shaped and irregularly spaced incremental lines; and 3) numerous globular formations embedded in dentin or free in the pulp. These results indicate that CsA induces abnormal mineralized matrix formation in dentin and in the peripheral part of the pulp in rat molars.
环孢素A（CsA）是一种广泛使用的免疫抑制药物，可导致牙龈增生和骨重塑改变。本研究的目的是探讨CsA对牙本质可能产生的影响。将30mg/kg/天的CsA口服给予雄性Sprague-Dawley大鼠，持续19周。相同数量的对照大鼠接受油基赋形剂溶液。对大鼠实施麻醉，通过心脏内灌注固定液对组织进行固定。解剖下颌骨，脱矿，并进行环氧树脂包埋处理。第一磨牙的半薄切片显示，在六只实验动物中有四只的继发性牙本质-牙髓界面出现了改变。这些改变包括形成：1）骨样牙本质刺，其体积以及与继发性牙本质的界面面积分别约为25,000至75,000立方微米和1400至3530平方微米；2）形状异常且间距不规则的生长线；3）大量球状结构，嵌入牙本质或游离于牙髓中。这些结果表明，CsA可诱导大鼠磨牙的牙本质和牙髓周边部分形成异常矿化基质。
