Lovschall H, Fejerskov O, Flyvbjerg A
Department of Dental Pathology, Operative Dentistry and Endodontics, Royal Dental College, Faculty of Health Sciences, University of Aarhus, Vennelyst Boulevard 9, DK-8000 Aarhus C, Denmark.
Adv Dent Res. 2001 Aug;15:108-12. doi: 10.1177/08959374010150010301.
The aim of this study was to explore pulp healing and reparative dentinogenesis following pulp-capping by using recombinant human insulin-like growth factor I (rhIGF-I). Exposures were made through the mesial pulp horn in first upper molars in two-month-old Wistar rats. The pulp was covered with one dose of sterile 4% methylcellulose gel containing either 400 ng rhIGF-I or saline in contralateral controls. The exposure site was closed with sterile Teflon membrane, and the cavity was filled with IRM cement. Additional molars were capped with Dycal as controls. After 3, 7, or 28 days, animals were anesthetized and fixed by intravascular glutaraldehyde perfusion. Molars were decalcified and processed for histological analysis and cut with membrane and residual methacrylate from IRM in situ. Only specimens with acceptable pulp sealing according to blinded microscopy control were included. On day 3, identical inflammatory responses in the upper pulp were observed in molars with rhIGF-I gel or control gel. On day 7, granulation tissue ingrowth had partly replaced inflammatory infiltration in both groups. After 28 days, complete dentin bridging and tubular dentin formation were observed more frequently and closer to the test substance containing rhIGF-I. The reparative dentin response to capping with rhIGF-I was similar to that after the use of Dycal. In conclusion, microscopic control of membrane sealing in situ gives valid information on the more subtle pulp effects of growth factors. The observations suggest that pulp-capping of rat molars by means of rhIGF-I enhances reparative dentinogenesis in comparison with vehicle controls.
本研究的目的是探讨使用重组人胰岛素样生长因子I（rhIGF-I）进行盖髓后牙髓愈合和修复性牙本质形成情况。在2月龄Wistar大鼠的上颌第一磨牙近中髓角处进行牙髓暴露。在对侧对照组中，用一剂含400 ng rhIGF-I的无菌4%甲基纤维素凝胶或生理盐水覆盖牙髓。用无菌聚四氟乙烯膜封闭暴露部位，并用IRM粘固粉充填窝洞。另外的磨牙用Dycal盖髓作为对照。在3、7或28天后，将动物麻醉并通过血管内戊二醛灌注固定。磨牙脱钙后进行组织学分析，原位切割去除膜和IRM中的残留甲基丙烯酸酯。仅纳入根据盲法显微镜检查控制具有可接受牙髓封闭的标本。在第3天，在使用rhIGF-I凝胶或对照凝胶的磨牙中，观察到上颌牙髓的炎症反应相同。在第7天，两组中肉芽组织向内生长部分取代了炎症浸润。28天后，更频繁地观察到完全的牙本质桥接和管状牙本质形成，且更靠近含rhIGF-I的测试物质。rhIGF-I盖髓后的修复性牙本质反应与使用Dycal后的相似。总之，原位对膜封闭进行显微镜控制可提供关于生长因子对牙髓更细微影响的有效信息。观察结果表明，与载体对照相比，用rhIGF-I对大鼠磨牙进行盖髓可增强修复性牙本质形成。
