Rath P M, Ratjen F, Ansorg R
Institut für Medizinische Mikrobiologie, Universität-GH Essen, Germany.
Zentralbl Bakteriol. 1997 Feb;285(3):450-5. doi: 10.1016/s0934-8840(97)80012-6.
Strains of Aspergillus fumigatus (n = 24) were isolated from the sputa of six patients with cystic fibrosis during periods from 3 to 11 months. The genetic polymorphisms of the strains were studied using the random amplified polymorphic DNA (RAPD) assay with three single oligonucleotides and pairwise combined primers. The analysis of RAPD patterns resulted in 15 different RAPD types. In four patients, the colonizing type changed, whereas in two others the same types were detected over periods between 3 and 11 months. The genetic diversity as well as the shift of the colonizing strains found in some patients might be important for the epidemiology of Aspergillus infections in patients with cystic fibrosis.
在3至11个月期间，从6例囊性纤维化患者的痰液中分离出烟曲霉菌株（n = 24）。使用三种单链寡核苷酸和两两组合引物的随机扩增多态性DNA（RAPD）分析法研究了这些菌株的基因多态性。RAPD图谱分析产生了15种不同的RAPD类型。在4例患者中，定植类型发生了变化，而在另外2例患者中，在3至11个月期间检测到相同的类型。在一些患者中发现的定植菌株的遗传多样性以及变化可能对囊性纤维化患者曲霉感染的流行病学具有重要意义。
