Neuvéglise C, Sarfati J, Debeaupuis J P, Vu Thien H, Just J, Tournier G, Latgé J P
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
Eur J Clin Microbiol Infect Dis. 1997 Oct;16(10):747-50. doi: 10.1007/BF01709257.
The colonization over time of cystic fibrosis patients by Aspergillus fumigatus was investigated using a DNA fingerprinting method. Aspergillus fumigatus isolates collected sequentially for more than one year from six patients with cystic fibrosis were typed by Southern blot hybridization with a repetitive DNA sequence. Each cystic fibrosis patient harbored several strains of Aspergillus fumigatus that were isolated recurrently over time. Isolates collected from a cystic fibrosis patient with aspergilloma displayed the same genotype, suggesting that the infection was due to a single strain. Continuous isolation of the same genotype in another cystic fibrosis patient, however, was not correlated clinically with an Aspergillus infection.
采用DNA指纹图谱法研究了烟曲霉对囊性纤维化患者的长期定植情况。通过用重复DNA序列进行Southern印迹杂交，对从6例囊性纤维化患者连续收集一年多的烟曲霉分离株进行分型。每位囊性纤维化患者体内都携带数株烟曲霉，这些菌株随时间推移反复被分离出来。从患有曲霉菌瘤的囊性纤维化患者身上分离出的菌株显示出相同的基因型，这表明感染是由单一菌株引起的。然而，在另一位囊性纤维化患者中持续分离出相同基因型的菌株，在临床上与烟曲霉感染并无关联。
