Vikjaer D, Blom S, Hjørting-Hansen E, Pinholt E M
Department of Oral and Maxillofacial Surgery, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
Eur J Oral Sci. 1997 Feb;105(1):59-66. doi: 10.1111/j.1600-0722.1997.tb00181.x.
The effect of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on bone healing was examined in calvarial defects in rabbits. Bicortical circular (critical size) defects were prepared in the calvarial bone of 16 rabbits. The defects were closed on the dural side and covered externally with expanded polytetrafluoroethylene membranes to prevent interference with osteogenesis within the defect by the surrounding tissue and to keep the growth factor in place. A single dose of methylcellulose gel (4.4%) with (n = 8) or without rhPDGF-BB (50 micrograms/ml) (n = 8) was applied to the defects, and the bone formation was evaluated after 8 weeks. Healing of defects in both groups was characterized by the presence of newly formed bone along the edges of the original defect and by a central area of fibrous connective tissue. The newly formed bone in the rhPDGF-BB treated defects had a trabecular structure; in contrast, a more compact structure was found in the control defects. In the rhPDGF-BB-treated defects, the bone ingrowth was 51.8 +/- 7.1% compared to 30.5 +/- 3.3% in the control defects. Furthermore, the amount of mineralized tissue was increased 112% in the rhPDGF-BB group. The amount of bone marrow was increased 75% in the rhPDGF-BB-treated defect. The porosity of cortical lamella in the newly formed bone was 84% higher in the rhPDGF-BB-treated defects compared to the control. These results show that administration of a single dose of rhPDGF-BB stimulates bone formation in critical size calvarial defects.
在兔颅骨缺损模型中研究了重组人血小板衍生生长因子-BB（rhPDGF-BB）对骨愈合的影响。在16只兔的颅骨上制备双皮质圆形（临界尺寸）缺损。缺损在硬脑膜侧封闭，外部用膨体聚四氟乙烯膜覆盖，以防止周围组织干扰缺损内的骨生成，并使生长因子保持在原位。将单剂量含（n = 8）或不含rhPDGF-BB（50微克/毫升）（n = 8）的甲基纤维素凝胶（4.4%）应用于缺损处，8周后评估骨形成情况。两组缺损的愈合均表现为原始缺损边缘有新形成的骨以及中央有纤维结缔组织区域。rhPDGF-BB处理的缺损中新形成的骨具有小梁结构；相比之下，对照缺损中的结构更致密。在rhPDGF-BB处理的缺损中，骨长入为51.8±7.1%，而对照缺损中为30.5±3.3%。此外，rhPDGF-BB组矿化组织量增加了112%。rhPDGF-BB处理的缺损中骨髓量增加了75%。与对照相比，rhPDGF-BB处理的缺损中新形成骨的皮质板孔隙率高84%。这些结果表明，单次给予rhPDGF-BB可刺激临界尺寸颅骨缺损中的骨形成。
