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2
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本文引用的文献

1
Synthesis and Posttranslational Activation of Sulfhydryl-Endopeptidase in Cotyledons of Germinating Vigna mungo Seeds.硫醇内切酶在绿豆种子萌发子叶中的合成和翻译后激活。
Plant Physiol. 1989 Jan;89(1):274-9. doi: 10.1104/pp.89.1.274.
2
Fluorescence immunohistochemical localization of malate dehydrogenase isoenzymes in watermelon cotyledons : a developmental study of glyoxysomes and mitochondria.荧光免疫组织化学定位法在西瓜子叶中苹果酸脱氢酶同工酶的研究：乙醛酸体和线粒体的发育研究。
Plant Physiol. 1982 Oct;70(4):1162-8. doi: 10.1104/pp.70.4.1162.
3
Organelle-bound malate dehydrogenase isoenzymes are synthesized as higher molecular weight precursors.细胞器结合的苹果酸脱氢酶同工酶作为高分子量前体合成。
Plant Physiol. 1982 Aug;70(2):483-7. doi: 10.1104/pp.70.2.483.
4
Microbodies and the problem of mitochondrial regeneration in liver cells.微体与肝细胞中线粒体再生的问题。
J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):355-60. doi: 10.1083/jcb.2.4.355.
5
Glyoxysomal malate dehydrogenase and malate synthase from soybean cotyledons (Glycine max L.): enzyme association, antibody production and cDNA cloning.大豆子叶（Glycine max L.）中的乙醛酸循环体苹果酸脱氢酶和苹果酸合酶：酶的关联、抗体产生及cDNA克隆
Planta. 1995;197(2):369-75. doi: 10.1007/BF00202659.
6
Protein import into peroxisomes and biogenesis of the organelle.蛋白质导入过氧化物酶体与该细胞器的生物发生
Annu Rev Cell Biol. 1993;9:445-78. doi: 10.1146/annurev.cb.09.110193.002305.
7
Mutational analysis of the N-terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha.利用异源宿主多形汉逊酵母对西瓜乙醛酸循环体苹果酸脱氢酶N端拓扑信号进行突变分析。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3151-5. doi: 10.1073/pnas.91.8.3151.
8
Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo.酿酒酵母3-酮酰基辅酶A硫解酶氨基靶向信号的诱变揭示了体内导入过氧化物酶体所需的保守氨基酸。
J Biol Chem. 1994 Mar 11;269(10):7558-63.
9
Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor.大鼠过氧化物酶体3-酮酰基辅酶A硫解酶前体氨基末端信号肽的特征分析。
J Biol Chem. 1994 Feb 25;269(8):6001-10.
10
Thiolase mRNA translated in vitro yields a peptide with a putative N-terminal presequence.硫解酶信使核糖核酸在体外翻译产生一种带有推定的N端前序列的肽。
Plant Mol Biol. 1993 Apr;22(1):59-66. doi: 10.1007/BF00038995.

从蓖麻籽胚乳微体中分离出的一种半胱氨酸内肽酶可加工乙醛酸循环体苹果酸脱氢酶前体蛋白。

A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein.

作者信息

Gietl C, Wimmer B, Adamec J, Kalousek F

机构信息

Institute of Botany, Technical University of Munich, Germany.

出版信息

Plant Physiol. 1997 Mar;113(3):863-71. doi: 10.1104/pp.113.3.863.

DOI:10.1104/pp.113.3.863
PMID:9085576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC158206/
Abstract

A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first intermediate resulting from cleavage after arginine-13 within the presequence and the second from cleavage after arginine-33. The endopeptidase is unable to remove the presequences of prethiolases from rape (Brassica napus) glyoxysomes and rat peroxisomes at the expected cleavage site. Protein sequence analysis of N-terminal and internal peptides revealed high identity to the mature papain-type cysteine endopeptidases from cotyledons of germinating mung bean (Vigna mungo) and French bean (Phaseolus vulgaris) seeds. These endopeptidases are synthesized with an extended pre-/prosequence at the N terminus and have been considered to be processed in the endoplasmic reticulum and targeted to protein-storing vacuoles.

摘要

从萌发蓖麻（Ricinus communis）胚乳的微体中纯化出一种分子量为35 kD的植物半胱氨酸内肽酶，其依据在于它能够在体外将乙醛酸循环体苹果酸脱氢酶前体蛋白特异性加工成成熟亚基。乙醛酸循环体苹果酸脱氢酶前体的加工依次分三步进行，第一个中间体是在前导序列内精氨酸-13之后切割产生的，第二个中间体是在精氨酸-33之后切割产生的。该内肽酶无法在预期切割位点去除油菜（Brassica napus）乙醛酸循环体和大鼠过氧化物酶体中硫解酶前体的前导序列。对N端和内部肽段的蛋白质序列分析表明，它与萌发绿豆（Vigna mungo）和菜豆（Phaseolus vulgaris）种子子叶中成熟的木瓜蛋白酶型半胱氨酸内肽酶具有高度同源性。这些内肽酶在N端合成时带有一个延长的前导/前体序列，并且被认为在内质网中进行加工并靶向到蛋白质储存液泡。