源自SHV-5变体的耐抑制剂β-内酰胺酶（SHV-10）的出现。
Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant.
作者信息
Prinarakis E E, Miriagou V, Tzelepi E, Gazouli M, Tzouvelekis L S
机构信息
Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
出版信息
Antimicrob Agents Chemother. 1997 Apr;41(4):838-40. doi: 10.1128/AAC.41.4.838.
Abstract
An inhibitor-resistant beta-lactamase (SHV-10), derived from an SHV-5 variant (SHV-9), was found in an Escherichia coli clinical isolate. In SHV-10, Ser-130 was replaced by Gly. The enzyme partially retained its ability to hydrolyze penicillins, but its activity against cephalosporins was drastically reduced. A Ser-130-->Gly mutant of the prototype SHV-5, obtained by site-directed mutagenesis, had properties similar to those of SHV-10.
摘要
在一株大肠杆菌临床分离株中发现了一种源自SHV-5变体（SHV-9）的耐抑制剂β-内酰胺酶（SHV-10）。在SHV-10中，丝氨酸-130被甘氨酸取代。该酶部分保留了水解青霉素的能力，但其对头孢菌素的活性大幅降低。通过定点诱变获得的原型SHV-5的丝氨酸-130→甘氨酸突变体具有与SHV-10相似的特性。
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