Albee R R, Nitschke K D, Mattsson J L, Stebbins K E
Dow Chemical Company, Midland, MI 48674, USA.
Neurotoxicol Teratol. 1997 Jan-Feb;19(1):27-37. doi: 10.1016/s0892-0362(96)00182-1.
Humans overexposed to trichloroethylene (TCE), under specific conditions, were reported to develop trigeminal nerve dysfunction. A degradation byproduct dichloroacetylene (DCA), however, has been suggested as the probable neurotoxicant rather than TCE. Studies in mice, rats, and rabbits support the hypothesis of DCA-induced trigeminal neurotoxicity. This study, therefore, was conducted to characterize DCA-induced trigeminal nerve dysfunction in rats using the electrodiagnostic procedure trigeminal nerve-stimulated somatosensory evoked potential (TSEP). A group of six rats was exposed once to DCA (approximately 300 ppm) or room air for 2.25 h and a separate group of six rats was not exposed and served as controls. Trigeminal nerve somatosensory evoked potentials (TSEPs) were collected before exposure and 2, 4, and 7 days postexposure. Because DCA was manufactured from TCE with acetylene added as a stabilizer, another group of rats was exposed to TCE and acetylene without generation of DCA. TSEPs from DCA-exposed rats were smaller and slower compared to their baseline recordings and to the concurrent negative controls. TSEPs from the controls and the TCE/acetylene-exposed rats were unchanged. Neuropathology did not reveal treatment-related lesions. It was concluded that the rat is mildly to markedly susceptible to DCA-induced trigeminal nerve dysfunction as assessed by TSEP, but that the kidney was the likely target organ based on gross observations and the DCA literature.
据报道，在特定条件下，过度接触三氯乙烯（TCE）的人会出现三叉神经功能障碍。然而，一种降解副产物二氯乙炔（DCA）被认为可能是神经毒素，而非TCE。对小鼠、大鼠和兔子的研究支持了DCA诱导三叉神经毒性的假说。因此，本研究采用电诊断程序三叉神经刺激体感诱发电位（TSEP）来表征DCA诱导的大鼠三叉神经功能障碍。一组六只大鼠单次暴露于DCA（约300 ppm）或室内空气中2.25小时，另一组六只未暴露的大鼠作为对照。在暴露前以及暴露后2、4和7天收集三叉神经体感诱发电位（TSEP）。由于DCA是由添加乙炔作为稳定剂的TCE制造而成，另一组大鼠暴露于TCE和乙炔中，但未生成DCA。与基线记录和同期阴性对照相比，暴露于DCA的大鼠的TSEP更小且更慢。对照组和暴露于TCE/乙炔的大鼠的TSEP没有变化。神经病理学未发现与治疗相关的病变。得出的结论是，根据TSEP评估，大鼠对DCA诱导的三叉神经功能障碍有轻度至明显的易感性，但基于大体观察和DCA文献，肾脏可能是靶器官。
