AuBuchon J P, Elfath M D, Popovsky M A, Stromberg R R, Pickard C, Herschel L, Whitley P, McNeil D, Arnold N, O'Connor J L
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, N.H. 03756, USA.
Vox Sang. 1997;72(2):101-6. doi: 10.1046/j.1423-0410.1997.7220101.x.
Prestorage leukoreduction offers a variety of potential benefits and is becoming more commonly practiced. The LeukoNet prestorage leukoreduction filtration system is intended for leukoreduction of red blood cells and uses a vent to allow automatic drainage of red cells from the filter.
We studied the functional characteristics and the in-vivo and in-vitro properties of leukoreduced AS-1 Red Blood Cells prepared with this new system. Units of AS-1 Red Blood Cells were filtered at 4 degrees C through the LeukoNet filter 24-48 h after collection and stored under usual conditions for 42 days. Residual leukocytes were enumerated using a Nageotte chamber or with a polymerase chain reaction (PCR) technique. In the clinical trial (phase one), 21 donors had units stored with and without leukoreduction for 42 days; biochemical assays were done before and after storage, and 51Cr/99mTc red cell recovery studies at the end of the storage period.
Leukocyte content after filtration was 3.2 +/- 2.6 x 10(4)/unit (n = 21), and all units had < 1 x 10(5) leukocytes (median: 3.8 x 10(4)). In-vivo paired studies showed no difference in 24-hour recovery (control: 82.1 +/- 5.8%; test: 82.9 +/- 6.0%). Hemolysis was halved with leuko-reduction (0.59 +/- 0.30 vs. 0.29 +/- 0.11%; p < 0.05), and glucose consumption was reduced by 5% compared to control units (p = < 0.05). Other biochemical parameters showed no differences. In the practical trial (phase two), filtration time was 41 +/- 23 min. With a residual leukocyte content of 6.6 +/- 4.9 x 10(4)/unit and 14 +/- 3% red cell loss (n = 84). Six additional units underwent leukocyte enumeration by PCR and had 2.6 +/- 1.1 x 10(4) residual leukocytes.
Under the conditions studied, the LeukoNet leukoreduction filtration system produces about 4-5 log10 leukocyte content reduction.
储存前白细胞滤除具有多种潜在益处，且应用日益普遍。LeukoNet储存前白细胞滤除过滤系统旨在对红细胞进行白细胞滤除，并通过一个排气孔实现红细胞从过滤器的自动引流。
我们研究了用这种新系统制备的白细胞滤除后的AS-1红细胞的功能特性以及体内和体外性质。AS-1红细胞单位在采集后24 - 48小时于4℃通过LeukoNet过滤器进行过滤，并在常规条件下储存42天。使用纳盖特计数板或聚合酶链反应（PCR）技术对残留白细胞进行计数。在临床试验（第一阶段）中，21名供者的血液单位分别储存有和没有进行白细胞滤除的样本42天；在储存前后进行生化检测，并在储存期结束时进行51Cr/99mTc红细胞回收率研究。
过滤后的白细胞含量为3.2±2.6×10(4)/单位（n = 21），所有单位的白细胞均<1×10(5)（中位数：3.8×10(4)）。体内配对研究显示24小时回收率无差异（对照组：82.1±5.8%；试验组：82.9±6.0%）。白细胞滤除后溶血减半（0.59±0.30对0.29±0.11%；p < 0.05），与对照单位相比葡萄糖消耗降低了5%（p = < 0.05）。其他生化参数无差异。在实际试验（第二阶段）中，过滤时间为41±23分钟。残留白细胞含量为6.6±4.9×10(4)/单位，红细胞损失率为14±3%（n = 84）。另外6个单位通过PCR进行白细胞计数，残留白细胞为2.6±1.1×10(4)。
在所研究条件下，LeukoNet白细胞滤除过滤系统使白细胞含量降低约4至5个对数10。
