Doizaki W M, Zieve L
J Lab Clin Med. 1977 Nov;90(5):849-55.
An improved method for measuring MT in the blood is described which uses zinc as a reducing agent and utilizes a more sensitive gas chromatograph. The method measures covalently bound MT that was not previously detected and permits determinations in patients for the first time. The average normal whole blood MT in rat blood was 200 +/- 42 pmol/ml and in human blood 229 +/- 29 pmol/ml. Eleven rats in experimental hepatic coma following acute massive hepatic necrosis had blood levels four to 18 times the average normal, and seven cirrhotic patients with hepatic encephalopathy or coma had blood levels four to five times the average normal.
描述了一种改进的测量血液中金属硫蛋白（MT）的方法，该方法使用锌作为还原剂，并采用了更灵敏的气相色谱仪。该方法可测量先前未检测到的共价结合的MT，并首次能够对患者进行测定。大鼠血液中正常全血MT的平均水平为200±42 pmol/ml，人类血液中为229±29 pmol/ml。11只急性大量肝坏死导致实验性肝昏迷的大鼠，其血液水平是正常平均值的4至18倍，7名患有肝性脑病或昏迷的肝硬化患者，其血液水平是正常平均值的4至5倍。
