Mourad K M, Edelhauser H F, Capone A, Lynn M J, Geroski D H
Department of Ophthalmology, School of Medicine, Emory University, Atlanta, GA, USA.
Curr Eye Res. 1997 Mar;16(3):239-43. doi: 10.1076/ceyr.16.3.239.15414.
To compare the abilities of balanced salt solution, BSS Plus and Hartmann's lactated Ringer's (HLR) solution to maintain the viability of retinal vascular endothelial cells (RVEC) in vitro.
Cultured retinal vascular endothelial cells were suspended in each irrigating solution for four hours. Viability was determined by trypan blue exclusion at 30 minute intervals. Regression analysis was used to determine the rate of viability loss. Additional studies were performed to determine the effectiveness of lactate in maintaining cell viability.
Retinal vascular endothelial cells lost viability at a greater rate (p < 0.001) in BSS (8.7%/hr) compared with BSS Plus (3.3%/hr). Cells in Hartmann's lactated Ringer's lost viability at a significantly lower rate (4.4%/hr) than retinal vascular endothelial cells in lactate-free Hartmann's lactated Ringer's solution (8.4%/hr). Lactate was as effective as glucose in preserving RVEC viability. By comparison, the viability of corneal endothelial cells was not effectively maintained by lactate. For these cells, BSS Plus was clearly superior to Hartmann's lactated Ringer's solution in maintaining viability.
BSS Plus and Hartmann's lactated Ringer's solution are both superior to balanced salt solution in maintaining retinal vascular endothelial cell viability. For retinal vascular endothelial cells, Hartmann's lactated Ringer's solution preserves cell viability as well as BSS Plus, since the retinal vascular endothelial cells, unlike corneal endothelial cells, can apparently utilize lactate as an energy source.
比较平衡盐溶液、BSS Plus和哈特曼氏乳酸林格氏液（HLR）在体外维持视网膜血管内皮细胞（RVEC）活力的能力。
将培养的视网膜血管内皮细胞悬浮于每种冲洗液中4小时。每隔30分钟通过台盼蓝排斥法测定细胞活力。采用回归分析确定活力丧失率。进行了额外的研究以确定乳酸在维持细胞活力方面的有效性。
与BSS Plus（3.3%/小时）相比，视网膜血管内皮细胞在BSS中活力丧失率更高（p < 0.001）（8.7%/小时）。哈特曼氏乳酸林格氏液中的细胞活力丧失率（4.4%/小时）明显低于无乳酸的哈特曼氏乳酸林格氏液中的视网膜血管内皮细胞（8.4%/小时）。乳酸在保存RVEC活力方面与葡萄糖效果相当。相比之下，乳酸不能有效维持角膜内皮细胞的活力。对于这些细胞，BSS Plus在维持活力方面明显优于哈特曼氏乳酸林格氏液。
在维持视网膜血管内皮细胞活力方面，BSS Plus和哈特曼氏乳酸林格氏液均优于平衡盐溶液。对于视网膜血管内皮细胞，哈特曼氏乳酸林格氏液在保存细胞活力方面与BSS Plus相当，因为与角膜内皮细胞不同，视网膜血管内皮细胞显然可以利用乳酸作为能量来源。
