Rees-Jones R W, Larsen P R
Metabolism. 1977 Nov;26(11):1213-8. doi: 10.1016/0026-0495(77)90113-5.
Triiodothyronine (T3) and thyroxine (T4) were measured by radioimmunoassay in Pronase hydrolysates of four lots each of 1- and 2-grain tablets of desiccated thyroid (Thyroid, Armour) and thyroglobulin (Proloid, Warner-Chilcott). The methodology used was verified by studies of tablets containing known quantities of T4 and T3. One grain of desiccated thyroid contained 12 +/- 1 and 64 +/- 3 microgram (mean +/- SD) of T3 and T4 per tablet, respectively (T4/T3 molar ratio, 4.3). A 1-grain tablet of thyroglobulin contained 16 +/- 2 and 55 +/- 5 microgram of T3 and T4, respectively with a T4/T3 ratio of 2.9. Two-grain tablets generally contained twice the quantity of T3 and T4 in the 1-grain preparations. The variation in T3 and T4 content between the four lots of each tablet strength for each product was 10% or less. These estimates of T3 and T4 content are 1.5- to 2-fold greater than those previously published. This difference probably results from the more sophisticated methodology now available which does not require chromatographic separation of T3 and T4 or iodometry. Using calculations based on published estimates of T4 and T3 absorption and of the T3/T4 potency ratio, it would appear that the T3 content of desiccated thyroid and thyroglobulin provide approximately 39% and 51%, respectively, of the thyromimetic activity of these two medications.
采用放射免疫分析法对四批1格令和2格令的干燥甲状腺片（甲状腺片，阿摩尔）及甲状腺球蛋白片（普罗loid，华纳-奇尔科特）的链霉蛋白酶水解产物中的三碘甲状腺原氨酸（T3）和甲状腺素（T4）进行了测定。通过对含有已知量T4和T3的片剂进行研究，验证了所使用的方法。每片1格令的干燥甲状腺片中分别含有12±1微克和64±3微克（平均值±标准差）的T3和T4（T4/T3摩尔比为4.3）。一片1格令的甲状腺球蛋白片中分别含有16±2微克和55±5微克的T3和T4，T4/T3比值为2.9。2格令的片剂中T3和T4的含量通常是1格令制剂中的两倍。每种产品的每种片剂规格的四批产品之间T3和T4含量的差异为10%或更低。这些T3和T4含量的估计值比之前公布的高1.5至2倍。这种差异可能是由于现在可用的更精密的方法，该方法不需要对T3和T4进行色谱分离或碘量法。根据已发表的T4和T3吸收估计值以及T3/T4效价比进行计算，干燥甲状腺和甲状腺球蛋白中的T3含量似乎分别提供了这两种药物甲状腺模拟活性的约39%和51%。
