Kaikkonen J, Nyyssönen K, Porkkala-Sarataho E, Poulsen H E, Metsä-Ketelä T, Hayn M, Salonen R, Salonen J T
Research Institute of Public Health, University of Kuopio, Finland.
Free Radic Biol Med. 1997;22(7):1195-202. doi: 10.1016/s0891-5849(96)00549-7.
Coenzyme Q10 (Q10) is supposed to be an important endogenous lipid-soluble antioxidant. We studied 60 healthy 46 +/- 7 (mean +/- SD)-year-old smoking men. They were randomized into three groups to receive oil-based or granular Q10 (90 mg/d) or placebo for 2 months. Oil-based capsule elevated Q10 in plasma by 178% and in VLDL+LDL fraction by 160%. The granular preparation increased Q10 in plasma by 168% and in VLDL+LDL by 127%. However, the 2-month Q10 supplementation did not increase the oxidation resistance of VLDL+LDL fraction, as assessed by copper induced VLDL+LDL oxidation, haemin+H(2)O(2)-induced VLDL+LDL oxidation, total antioxidative capacity of LDL, and plasma malondialdehyde measurements. The first and the last dose was used to carry out a 12 h pharmacokinetic study (five subjects per group), which indicated that only a small part of supplemented Q10 was absorbed to the circulation in 12 h and that the absorption varied extensively between subjects. Our results suggest that at least among smoking men, 90 mg of orally supplemented Q10 daily does not increase the oxidation resistance of VLDL+LDL. Bioavailability of both the granular and the oil-based Q10 preparation was similar during the long-term supplementation, but one dose of 30 mg had only a marginal effect on the plasma levels of Q10.
辅酶Q10(Q10)被认为是一种重要的内源性脂溶性抗氧化剂。我们研究了60名健康的46±7(平均±标准差)岁吸烟男性。他们被随机分为三组,分别接受油基或颗粒状Q10(90毫克/天)或安慰剂,为期2个月。油基胶囊使血浆中的Q10升高了178%,使极低密度脂蛋白+低密度脂蛋白部分中的Q10升高了160%。颗粒制剂使血浆中的Q10升高了168%,使极低密度脂蛋白+低密度脂蛋白中的Q10升高了127%。然而,通过铜诱导的极低密度脂蛋白+低密度脂蛋白氧化、血红素+H₂O₂诱导的极低密度脂蛋白+低密度脂蛋白氧化、低密度脂蛋白的总抗氧化能力以及血浆丙二醛测量评估,为期2个月的Q10补充并未增加极低密度脂蛋白+低密度脂蛋白部分的抗氧化能力。使用首剂和末剂进行了一项12小时的药代动力学研究(每组5名受试者),结果表明,补充的Q10在12小时内仅有一小部分被吸收进入循环,而且个体之间的吸收差异很大。我们的结果表明,至少在吸烟男性中,每天口服补充90毫克Q10不会增加极低密度脂蛋白+低密度脂蛋白的抗氧化能力。在长期补充期间,颗粒状和油基Q10制剂的生物利用度相似,但一剂30毫克对Q10的血浆水平仅有微小影响。