插入元件IS 6110是用于对西班牙牛和山羊中分离出的牛分枝杆菌进行DNA指纹分析的有用工具。

The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain.

作者信息

Liébana E, Aranaz A, Dominguez L, Mateos A, González-Llamazares O, Rodriguez-Ferri E F, Domingo M, Vidal D, Cousins D

机构信息

Dpto. Patología Animal 1 (sanidad Animal), Facultad de Veterinaria UCM, Madrid, Spain.

出版信息

Vet Microbiol. 1997 Mar;54(3-4):223-33. doi: 10.1016/s0378-1135(96)01282-5.

DOI:10.1016/s0378-1135(96)01282-5

PMID:9100324

Abstract

A total of 129 Mycobacterium bovis strains from 5 different Spanish locations were fingerprinted using the IS6110 repetitive element. We demonstrated the presence of multiple copies (from 2 to 13) of IS6110 in a large proportion (47.4%) of the M.bovis strains isolated from cattle and we showed that these strains can be successfully differentiated by means of the RFLP with IS6110. All of the M. bovis strains isolated from goats had multiple copies of IS6110 and 4 bands of 2, 1.7, 1.4 and 1.3 kb were common in all the caprine RFLP patterns. The caprine strains formed a clearly separate cluster from the bovine strains.

摘要

使用IS6110重复元件对来自西班牙5个不同地点的总共129株牛分枝杆菌菌株进行了指纹分析。我们证明，从牛分离出的很大一部分（47.4%）牛分枝杆菌菌株中存在多个拷贝（2至13个）的IS6110，并且我们表明，这些菌株可以通过IS6110的限制性片段长度多态性（RFLP）成功区分。从山羊分离出的所有牛分枝杆菌菌株都有多个IS6110拷贝，并且在所有山羊RFLP图谱中，2 kb、1.7 kb、1.4 kb和1.3 kb的4条带是常见的。山羊菌株与牛菌株形成了一个明显独立的簇。