Szewzyk R, Svenson S B, Hoffner S E, Bölske G, Wahlström H, Englund L, Engvall A, Källenius G
Department of Bacteriology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
J Clin Microbiol. 1995 Dec;33(12):3183-5. doi: 10.1128/jcm.33.12.3183-3185.1995.
Forty-nine isolates of Mycobacterium bovis from humans and animals in Sweden were analyzed by restriction fragment length polymorphism (RFLP) patterns probed by the insertion element IS6110. Most isolates had patterns indicating the presence of only one or two genomic copies of the IS6110 insertion element. This simple type of pattern was found in all human isolates. In contrast, isolates from M. bovis infections in five herds of farmed deer in Sweden showed a specific RFLP pattern with seven bands, indicating seven copies of the IS6110 sequence. In 1958, Sweden was declared free from M. bovis in cattle. However, in 1987, M. bovis was reintroduced with imported farmed deer, and since 1991, 11 outbreaks in deer herds, but not in other livestock or wildlife, have been diagnosed. Continued RFLP studies of the new Swedish M. bovis isolates can reveal possible transmission of this deer strain to other animals or humans.
采用插入元件IS6110探针的限制性片段长度多态性（RFLP）模式，对瑞典人和动物身上分离出的49株牛分枝杆菌进行了分析。大多数分离株的模式表明仅存在一两个IS6110插入元件的基因组拷贝。所有人类分离株均发现了这种简单类型的模式。相比之下，瑞典五群养殖鹿的牛分枝杆菌感染分离株显示出具有七条带的特定RFLP模式，表明有七个IS6110序列拷贝。1958年，瑞典宣布牛群中无牛分枝杆菌。然而，1987年，随着进口养殖鹿再次引入了牛分枝杆菌，自1991年以来，已诊断出鹿群中有11起疫情，但其他家畜或野生动物中未出现。对瑞典新的牛分枝杆菌分离株继续进行RFLP研究，可以揭示这种鹿株向其他动物或人类的可能传播情况。
