Gutiérrez M, Samper S, Jiménez M S, van Embden J D, Marín J F, Martín C
Departamento de Patología Animal, Medicina Animal, Facultad de Veterinaria, Campus de Vegazana, León, Spain.
J Clin Microbiol. 1997 Dec;35(12):3328-30. doi: 10.1128/jcm.35.12.3328-3330.1997.
We have used spoligotyping to characterize 18 Mycobacterium bovis strains isolated from cattle and 23 M. bovis strains isolated from goats. The spoligotypes revealed that caprine strains form a separate and well-differentiated group that we refer to hereafter in this abstract as the caprine genotype. To evaluate the importance of this genotype as a cause of tuberculosis in other animal species, including humans, we applied the spoligotyping method to 112 strains, including to all isolates identified as M. bovis by a Mycobacterial National Reference Laboratory (Majadahonda, Madrid) from 1994 to 1996. Eighty-three of these strains were identified in human isolates. In addition to being identified in three goat isolates and two sheep isolates, the caprine genotype was also found in three isolates causing human tuberculosis. Evidence to support the argument that there is a zoonotic risk of caprine tuberculosis was presented by the identification of the caprine genotype in an isolate from a veterinary worker with a recent history of contact with tuberculous goats.
我们使用间隔寡核苷酸分型技术对从牛分离出的18株牛分枝杆菌菌株和从山羊分离出的23株牛分枝杆菌菌株进行了特征分析。间隔寡核苷酸分型显示，山羊菌株形成了一个独立且差异明显的群体，在本摘要中我们将其称为山羊基因型。为评估该基因型作为包括人类在内的其他动物物种结核病病因的重要性，我们将间隔寡核苷酸分型方法应用于112株菌株，其中包括1994年至1996年由国家分枝杆菌参考实验室（马德里马亚达翁达）鉴定为牛分枝杆菌的所有分离株。这些菌株中有83株在人类分离株中被鉴定出来。除了在3株山羊分离株和2株绵羊分离株中被鉴定出来外，在3株导致人类结核病的分离株中也发现了山羊基因型。从一名近期有接触结核病山羊病史的兽医工作者的分离株中鉴定出山羊基因型，为支持山羊结核病存在人畜共患病风险这一论点提供了证据。
