通过对引起牛和山羊结核病的牛分枝杆菌菌株进行分子分型来鉴别。
Differentiation by molecular typing of Mycobacterium bovis strains causing tuberculosis in cattle and goats.
作者信息
Gutiérrez M, Samper S, Gavigan J A, García Marín J F, Martín C
机构信息
Departamento Patología Animal: Medicina Animal, Facultad de Veterinaria, Universidad de Léon, Spain.
出版信息
J Clin Microbiol. 1995 Nov;33(11):2953-6. doi: 10.1128/jcm.33.11.2953-2956.1995.
Abstract
Forty Mycobacterium bovis isolates from cattle and goats were analyzed by using different repetitive genetic markers. The 23 M. bovis strains from goats were found to carry six to eight copies of the insertion sequence IS6110. In contrast, most of the bovine isolates contained only a single copy of this element. The standardized IS6110 fingerprinting by restriction fragment length polymorphism (RFLP), described for Mycobacterium tuberculosis strains, allowed the differentiation of caprine strains. Although this method was not useful for typing bovine isolates, the repetitive elements pTBN12 and DR proved to be suitable for this purpose. A procedure using PCR which amplifies IS6110 in the outward direction was found to be as sensitive as RFLP for typing M. bovis strains from goats. The use of PCR and RFLP methods based on the IS6110 polymorphism would be useful for epidemiological studies of caprine tuberculosis. The results are consistent with different strains of M. bovis being implicated in bovine and caprine tuberculosis.
摘要
使用不同的重复基因标记对来自牛和山羊的40株牛分枝杆菌分离株进行了分析。发现来自山羊的23株牛分枝杆菌菌株携带6至8个插入序列IS6110拷贝。相比之下，大多数牛分离株仅含有该元件的一个拷贝。针对结核分枝杆菌菌株描述的通过限制性片段长度多态性（RFLP）进行的标准化IS6110指纹图谱分析，能够区分山羊菌株。尽管该方法对牛分离株的分型无用，但重复元件pTBN12和DR被证明适用于此目的。发现一种使用PCR向外扩增IS6110的方法在对山羊来源的牛分枝杆菌菌株进行分型时与RFLP一样敏感。基于IS6110多态性的PCR和RFLP方法的使用将有助于山羊结核病的流行病学研究。结果表明，不同的牛分枝杆菌菌株与牛和山羊结核病有关。
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