Baba E, Ikemoto T, Fukata T, Sasai K, Arakawa A, McDougald L R
Department of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
Vet Microbiol. 1997 Mar;54(3-4):301-8. doi: 10.1016/s0378-1135(96)01289-8.
Chickens infected with Clostridium perfringens and Eimeria necatrix were examined bacteriologically and pathologically. When chickens were inoculated with 1.0 x 10(8) C. perfringens and/or 2 x 10(4) E. necatrix sporulated oocysts, populations of C. perfringens in the intestinal contents were examined on 3, 5 and 7 days after E. necatrix inoculation. In both groups infected with E. necatrix, the mean clostridial counts were significantly higher than those of uninfected controls. The concurrent infection had no enhancing effects on increasing the clostridial population more than E. necatrix-alone. Mortality of 4-day-old chickens inoculated on 5 consecutive days with C.perfringens after receiving E. necatrix was higher than those of chickens inoculated with the both organisms. However, intestinal lesions of the concurrently infected group were not different from E. necatrix-alone-infected group on 5 and 7 days after the coccidial infection. When chickens received a large dose (1.5 x 10(9)) of C. perfringens after the inoculation with E. necatrix, edema in the duodenum through jejunum were observed early after the bacterial broth inoculation. These results suggest that the concurrent infection with E. necatrix and C. perfringens increases clostridial population in the intestine of the chickens and has synergic effects on mortality and edema in the upper intestine.
对感染产气荚膜梭菌和毒害艾美耳球虫的鸡进行了细菌学和病理学检查。当给鸡接种1.0×10⁸产气荚膜梭菌和/或2×10⁴毒害艾美耳球虫子孢子化卵囊后，在接种毒害艾美耳球虫后的第3、5和7天检查肠道内容物中产气荚膜梭菌的数量。在两组感染毒害艾美耳球虫的鸡中，梭菌平均计数均显著高于未感染的对照组。与单独感染毒害艾美耳球虫相比，同时感染对增加梭菌数量没有增强作用。在感染毒害艾美耳球虫后连续5天接种产气荚膜梭菌的4日龄鸡的死亡率高于接种两种病原体的鸡。然而，在球虫感染后的第5和7天，同时感染组的肠道病变与单独感染毒害艾美耳球虫组没有差异。当鸡在接种毒害艾美耳球虫后接受大剂量（1.5×10⁹）的产气荚膜梭菌时，在接种细菌肉汤后早期观察到十二指肠至空肠出现水肿。这些结果表明，毒害艾美耳球虫和产气荚膜梭菌同时感染会增加鸡肠道中的梭菌数量，并对上部肠道的死亡率和水肿有协同作用。
