Kenarova B, Voinov L, Apostolov C, Vladimirova R, Misheva A
Military Medical Academy, Immunology Center, Sofia, Bulgaria.
Eur J Ophthalmol. 1997 Jan-Mar;7(1):64-7. doi: 10.1177/112067219700700112.
To determine the cytokine-mediated pathways of inflammation in the clinical course of proliferative vitreoretinopathy (PVR).
Cytokines IL-2, IL-6, INF-gamma were measured by enzyme-linked immunosorbent assays. Aspirates of subretinal fluid were obtained from 14 eyes with PVR and six with uncomplicated rhegmatogenous retinal detachments. Aspirates obtained via pars plana from ten cadaver vitreouses of normal eyes were used as controls.
Subretinal fluids from eyes with PVR, uncomplicated rhegmatogenous retinal detachment and cadaver vitreous contained low concentrations of IL-2, with inconsequential differences between groups. IL-6 and IFN-gamma were twice as high in the subretinal fluid from eyes with rhetmatogenous retinal detachment as in cadaver vitreous. INF-gamma was elevated up to six times in eyes with PVR compared to controls. Fibronectin was found in a larger proportion of eyes with PVR than with uncomplicated rhegmatogenous retinal detachment and cadaver vitreous from healthy persons.
These are only preliminary investigations and are too few for statistical analysis. The results suggest that cytokine-mediated pathways of inflammation are involved in the pathogenesis of rhegmatogenous retinal detachment and in cellular interactions leading to the development of PVR.
确定增殖性玻璃体视网膜病变（PVR）临床过程中细胞因子介导的炎症途径。
采用酶联免疫吸附测定法检测细胞因子白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、γ干扰素（INF-γ）。从14例PVR患眼和6例单纯孔源性视网膜脱离患眼中获取视网膜下液抽吸物。通过平坦部从10只正常眼的尸体玻璃体中获取的抽吸物用作对照。
PVR患眼、单纯孔源性视网膜脱离患眼和尸体玻璃体的视网膜下液中IL-2浓度较低，各组间差异不显著。孔源性视网膜脱离患眼视网膜下液中IL-6和IFN-γ的含量是尸体玻璃体中的两倍。与对照组相比，PVR患眼中IFN-γ升高至6倍。与单纯孔源性视网膜脱离患眼以及健康人的尸体玻璃体相比，PVR患眼中发现纤连蛋白的比例更高。
这些只是初步研究，样本数量太少，无法进行统计分析。结果表明，细胞因子介导的炎症途径参与了孔源性视网膜脱离的发病机制以及导致PVR发生的细胞间相互作用。
