Meryman H T, Hornblower M
Transfusion. 1977 Sep-Oct;17(5):438-42. doi: 10.1046/j.1537-2995.1977.17578014580.x.
Although the high (40 to 50 per cent) glycerol method of freezing red blood cells has many advantages, no feasible procedure for deglycerolizing with only a clinical centrifuge has been available. Thus this method has been restricted to blood centers that distribute a sufficient number of frozen cells to warrant the installation of automated cell washing equipment. However, by sedimenting the glycerolized cells prior to freezing and discarding the excess glycerol solution, postthaw deglycerolizing can be simplified to consist of an initial double dilution with hypertonic NaCl and isotonic saline-glucose followed by two cycles of sedimentation and resuspension in isotonic saline-glucose. The method is applicable to units frozen either at -80 C or in liquid nitrogen vapor. Units frozen in this way can also be deglycerolized in commercial automated cell washers using suitably modified protocols.
尽管采用高浓度（40%至50%）甘油冷冻红细胞的方法有诸多优点，但一直没有仅使用临床离心机进行甘油去除的可行程序。因此，这种方法仅限于那些分发足够数量的冷冻细胞以保证安装自动细胞洗涤设备的血库。然而，通过在冷冻前沉淀甘油化细胞并弃去多余的甘油溶液，解冻后的甘油去除可以简化为首先用高渗氯化钠和等渗盐水 - 葡萄糖进行双重稀释，然后在等渗盐水 - 葡萄糖中进行两轮沉淀和重悬。该方法适用于在-80°C或液氮蒸汽中冷冻的单位。以这种方式冷冻的单位也可以使用适当修改的方案在商业自动细胞洗涤器中进行甘油去除。
