Elghouzzi M H, Sabolic V, Cavalier J
Rev Fr Transfus Immunohematol. 1984 Feb;27(1):67-76. doi: 10.1016/s0338-4535(84)80007-0.
We describe here a freezing method with a final low concentration of 20% glycerol and storage in a aluminium flask at - 150 degrees C. This technique allows the freezing of red blood cells concentrates collected on CPD and resuspended in SAG, without having to eliminate the SAG solution. For this we used an initial solution of 60% glycerol, which we added to the red blood cell concentrate in two steps separated by a resting time. Accordingly to the final concentration of glycerol, this freezing process is similar to the method currently used in our laboratory. This consists in one step addition V/V of 40% glycerol solution to the CPD red blood cells. The two techniques have been compared after thawing, and washing in a IBM Blood Processor 2991. The following parameters have been studied mainly: --haemoglobin level --residual glycerol --leucocytes and platelets --amount of 2-3 DPG and of intra-erythrocyte ATP. The results of the two freezing techniques are similar. They show that the SAG solution does not impair the intracellular penetration of glycerol. The in vitro qualities of the erythrocytes are well preserved.
我们在此描述一种冷冻方法，其最终甘油浓度为20%，并储存在-150℃的铝瓶中。该技术能够冷冻收集在CPD中并重悬于SAG中的红细胞浓缩物，而无需去除SAG溶液。为此，我们使用了60%甘油的初始溶液，分两步添加到红细胞浓缩物中，两步之间有一段静置时间。根据甘油的最终浓度，这种冷冻过程与我们实验室目前使用的方法类似。后者是将40%甘油溶液以V/V方式一次性添加到CPD红细胞中。解冻后，在IBM血液处理器2991中进行洗涤，对这两种技术进行了比较。主要研究了以下参数：——血红蛋白水平——残余甘油——白细胞和血小板——2,3-二磷酸甘油酸（2,3-DPG）和红细胞内三磷酸腺苷（ATP）的含量。两种冷冻技术的结果相似。结果表明，SAG溶液不会妨碍甘油的细胞内渗透。红细胞的体外质量得到了很好的保存。
