Appelbaum F R, Trapani R J, Graw R G
Transfusion. 1977 Sep-Oct;17(5):460-4. doi: 10.1046/j.1537-2995.1977.17578014584.x.
The transfusion of leukocyte-containing blood products can lead to the production of antibodies to antigens on the surface of leukocytes. Such antibodies can be detected by a variety of techniques including assays for lymphocytotoxicity, granulocytotoxicity, and leukoagglutination. In order to evaluate the effect of preformed anti-leukocyte antibodies during granulocyte transfusion therapy, recipient beagles were sensitized to donor foxhound antigens. After being made granulocytopenic with cyclophosphamide, these animals were transfused with a set dose of granulocytes collected by continuous flow centrifugation. When compared to the results of similar transfusions to nonsensitized recipients, granulocyte transfusions to animals with preformed anti-leukocyte antibodies resulted in lower one-hour posttransfusion leukocyte increments (p less than .04) and in less migration of neutrophils through a skin abrasion into a chamber containing a strong chemoattractant, autologous serum (p less than .0001). Also, profound thrombocytopenia was found in sensitized animals, but not in nonsensitized recipients, one hour after the granulocyte transfusion.
输注含有白细胞的血液制品可导致产生针对白细胞表面抗原的抗体。此类抗体可通过多种技术检测，包括淋巴细胞毒性、粒细胞毒性和白细胞凝集检测。为了评估粒细胞输注治疗期间预先形成的抗白细胞抗体的作用，将受体比格犬对供体猎狐犬抗原进行致敏。在用环磷酰胺使其粒细胞减少后，给这些动物输注一定剂量通过连续流动离心收集的粒细胞。与对未致敏受体进行类似输注的结果相比，给预先形成抗白细胞抗体的动物输注粒细胞导致输血后1小时白细胞增加较低（p小于0.04），并且中性粒细胞通过皮肤擦伤迁移到含有强趋化剂自体血清的腔室中的迁移较少（p小于0.0001）。此外，在粒细胞输注1小时后，在致敏动物中发现严重血小板减少，但在未致敏受体中未发现。
