一种用于测定茶儿茶素的反相高效液相色谱法。
A RP-HPLC method for the determination of tea catechins.
作者信息
Khokhar S, Venema D, Hollman P C, Dekker M, Jongen W
机构信息
DLO-State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Wageningen, The Netherlands.
出版信息
Cancer Lett. 1997 Mar 19;114(1-2):171-2. doi: 10.1016/s0304-3835(97)04653-3.
An HPLC method with gradient elution for the quantification of catechins ((-)-epigallocatechin (EGC), (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechingallate (EGCg) and (-)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). (+)-Catechin was not present in any of these teas.
建立了一种采用梯度洗脱的高效液相色谱法，用于定量测定茶叶中的儿茶素（（-）-表没食子儿茶素（EGC）、（+）-儿茶素（C）、（-）-表儿茶素（EC）、（-）-表没食子儿茶素没食子酸酯（EGCg）和（-）-表儿茶素没食子酸酯（ECg））。该方法用于测定红茶、绿茶和乌龙茶中的儿茶素。样品制备简单，因为仅需过滤和调节pH值。不同茶叶中儿茶素的含量在<5至287（EGC）、17至94（EC）、18至408（EGCg）和8至85 mg/L（ECg）之间变化。这些茶叶中均不存在（+）-儿茶素。
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