Shida O, Takagi H, Kadowaki K, Nakamura L K, Komagata K
Research Laboratory, Higeta Shoyu Co., Ltd., Chiba, Japan.
Int J Syst Bacteriol. 1997 Apr;47(2):289-98. doi: 10.1099/00207713-47-2-289.
We determined the taxonomic status of six Bacillus species (Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus) by using the results of 16S rRNA gene sequence and cellular fatty acid composition analyses. Phylogenetic analysis clustered these species closely with the Paenibacillus species. Like the Paenibacillus species, the six Bacillus species contained anteiso-C15:0 fatty acid as a major cellular fatty acid. The use of a specific PCR primer designed for differentiating the genus Paenibacillus from other members of the Bacillaceae showed that the six Bacillus species had the same amplified 16S rRNA gene fragment as members of the genus Paenibacillus. Based on these observations and other taxonomic characteristics, the six Bacillus species were transferred to the genus Paenibacillus. In addition, we propose emendation of the genus Paenibacillus.
我们通过16S rRNA基因序列和细胞脂肪酸组成分析的结果，确定了六种芽孢杆菌属物种（解藻酸芽孢杆菌、解软骨素芽孢杆菌、解凝胶多糖芽孢杆菌、解葡聚糖芽孢杆菌、小泉芽孢杆菌和解硫胺素芽孢杆菌）的分类地位。系统发育分析将这些物种与类芽孢杆菌属物种紧密聚类在一起。与类芽孢杆菌属物种一样，这六种芽孢杆菌属物种含有anteiso-C15:0脂肪酸作为主要细胞脂肪酸。使用为区分类芽孢杆菌属与芽孢杆菌科其他成员而设计的特异性PCR引物，结果表明这六种芽孢杆菌属物种具有与类芽孢杆菌属成员相同的扩增16S rRNA基因片段。基于这些观察结果和其他分类特征，这六种芽孢杆菌属物种被转移到类芽孢杆菌属。此外，我们提议对类芽孢杆菌属进行修订。
