Suzuki K, Sasaki J, Uramoto M, Nakase T, Komagata K
Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Int J Syst Bacteriol. 1997 Apr;47(2):474-8. doi: 10.1099/00207713-47-2-474.
"Curtobacterium psychrophilum," proposed by Inoue and Komagata in 1976, is a psychrophilic gram-positive irregular rod isolated from Antarctic soil. This organism grew optimally at 9 to 12 degrees C and did not grow at higher than 18 degrees C. Chemotaxonomic characteristics of this organism were the presence of 2,4-diaminobutyric acid in the cell wall and menaquinone-10 as the predominant respiratory quinone. The cellular fatty acid profile, which contained a significant amount of an anteiso-branched monounsaturated acid, 12-methyl tetradecenoic acid, was a distinctive characteristic of this organism and was reasonable for adaptation to low temperature. Phylogenetic analysis based on 16S ribosomal DNA sequences revealed that this organism was positioned at a separate branch in the family Microbacteriaceae, actinomycetes with group B peptidoglycan. We propose the name Cryobacterium psychrophilum gen. nov., sp. nov. for this organism. The type strain is JCM 1463 (= IAM 12024 = ATCC 43563 = IFO 15735 = NCIMB 2068).
1976年井上和驹形提出的“嗜冷短小杆菌”，是一种从南极土壤中分离出的嗜冷革兰氏阳性不规则杆菌。该微生物在9至12摄氏度下生长最佳，在高于18摄氏度时不生长。该微生物的化学分类特征是细胞壁中存在2,4-二氨基丁酸，以及以甲基萘醌-10作为主要呼吸醌。细胞脂肪酸谱含有大量的anteiso-支链单不饱和酸，12-甲基十四碳烯酸，是该微生物的一个显著特征，也是其适应低温的合理表现。基于16S核糖体DNA序列的系统发育分析表明，该微生物位于微杆菌科的一个单独分支上，该科是具有B组肽聚糖的放线菌。我们为此微生物提议新属名嗜冷杆菌属，新种名嗜冷嗜冷杆菌。模式菌株是JCM 1463（=IAM 12024=ATCC 43563=IFO 15735=NCIMB 2068）。
