Gould E, Cameron H A
Laboratory of Neuroendocrinology, Rockefeller University.
Behav Neurosci. 1997 Feb;111(1):49-56. doi: 10.1037//0735-7044.111.1.49.
These studies were conducted to determine whether (a) early N-methyl-D-aspartate (NMDA) receptor blockade impairs defensive behavior and (b) a relationship exists between defensive behavior and the production of granule cells in the dentate gyrus. Rat pups were treated with different doses of the NMDA receptor antagonist CGP 43487 on postnatal day (P) 5, and their behavior was observed following exposure to an unfamiliar adult male rat, a potential predator, on P13, P20, and P30. A dose-dependent impairment in freezing behavior was observed in rat pups treated with NMDA receptor antagonist on P13, P20, but not P30. Moreover, a dose-dependent increase in the number of (3)H-thymidine-labeled cells in the dentate gyrus was detected following CGP 43487 treatment, suggesting that an inverse relationship exists between cell proliferation and freezing behavior in rat pups following NMDA receptor blockade.
(a) 早期N-甲基-D-天冬氨酸（NMDA）受体阻断是否会损害防御行为；(b) 防御行为与齿状回颗粒细胞生成之间是否存在关联。在出生后第5天（P5），用不同剂量的NMDA受体拮抗剂CGP 43487处理幼鼠，并在出生后第13天（P13）、第20天（P20）和第30天（P30）让它们暴露于一只陌生成年雄性大鼠（潜在捕食者）后观察其行为。在P13和P20接受NMDA受体拮抗剂处理的幼鼠中观察到了冻结行为的剂量依赖性损害，但在P30未观察到。此外，CGP 43487处理后，在齿状回中检测到（3）H-胸腺嘧啶核苷标记细胞数量呈剂量依赖性增加，这表明NMDA受体阻断后，幼鼠的细胞增殖与冻结行为之间存在负相关关系。
