用灭活的gp120缺失型HIV-1免疫原免疫对HIV-1感染受试者β趋化因子和细胞因子产生的影响。
Effect of immunization with an inactivated gp120-depleted HIV-1 immunogen on beta-chemokine and cytokine production in subjects with HIV-1 infection.
作者信息
Moss R B, Trauger R J, Giermakowska W K, Turner J L, Wallace M R, Jensen F C, Richieri S P, Ferre F, Daigle A E, Duffy C, Theofan G, Carlo D J
机构信息
Immune Response Corporation, Carlsbad, California 92008, USA.
出版信息
J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Apr 1;14(4):343-50. doi: 10.1097/00042560-199704010-00006.
OBJECTIVE
To measure beta-chemokine and cytokine production in HIV-1-infected subjects undergoing treatment with HIV-1 immunogen (REMUNE).
DESIGN
Open label treatment study.
METHODS
beta-Chemokine and cytokine production in peripheral blood mononuclear cell (PBMC) culture.
RESULTS
Interferon-gamma production (p = 0.04) and lymphocyte proliferation (p = 0.001) to HIV-1 antigen-stimulated PBMCs increased after immunization with the HIV-1 immunogen. A correlation was demonstrated after immunization between HIV-1 antigen-stimulated lymphocyte proliferation and interferon-gamma levels (r = 0.53, p = 0.04). No significant change after immunization was seen for interleukin-4 production. A significant increase in mean levels of HIV-1 antigen-stimulated RANTES (i.e., regulated upon, activation normal T-cell expressed and secreted), was evident 1 month after immunization (p = 0.002) and remained elevated 3 months after immunization. RANTES production was decreased in CD8-depleted PBMC cultures. Mean serum HIV-1 RNA copy numbers and CD4 cell counts remained stable after immunization (p > 0.5). A correlation was demonstrated between HIV-1 antigen-stimulated interferon-gamma and RANTES production (r = 0.54, p = 0.002).
CONCLUSIONS
This report describes an augmentation of beta-chemokines and TH1-type cytokines from PBMCs after immunization with the HIV-1 immunogen.
目的
测定接受HIV-1免疫原（REMUNE）治疗的HIV-1感染受试者体内β趋化因子和细胞因子的产生情况。
设计
开放标签治疗研究。
方法
外周血单个核细胞（PBMC）培养中β趋化因子和细胞因子的产生情况。
结果
用HIV-1免疫原免疫后，HIV-1抗原刺激的PBMC产生的干扰素-γ（p = 0.04）和淋巴细胞增殖（p = 0.001）增加。免疫后，HIV-1抗原刺激的淋巴细胞增殖与干扰素-γ水平之间存在相关性（r = 0.53，p = 0.04）。免疫后白细胞介素-4的产生未见明显变化。免疫后1个月，HIV-1抗原刺激的RANTES（即调节激活正常T细胞表达和分泌）平均水平显著升高（p = 0.002），免疫后3个月仍保持升高。在CD8缺失的PBMC培养物中RANTES的产生减少。免疫后平均血清HIV-1 RNA拷贝数和CD4细胞计数保持稳定（p > 0.5）。HIV-1抗原刺激的干扰素-γ与RANTES产生之间存在相关性（r = 0.54，p = 0.002）。
结论
本报告描述了用HIV-1免疫原免疫后PBMC中β趋化因子和TH1型细胞因子的增加。
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