Reisner B S, Mann L M, Tholcken C A, Waite R T, Woods G L
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0740, USA.
J Clin Microbiol. 1997 May;35(5):1141-3. doi: 10.1128/jcm.35.5.1141-1143.1997.
The Captia Syphilis IgG enzyme immunoassay (EIA) was evaluated for use in conjunction with the rapid plasma reagin test (RPR) as a method to test for syphilis. A total of 1,288 serum specimens were tested by the routine laboratory protocol of the RPR followed by microhemagluttination assay for Treponema pallidum (MHA-TP) testing of RPR-reactive sera as well as the EIA-RPR protocol in which the automated EIA followed by a manual RPR test for EIA-positive specimens is used. When using the routine protocol, 131 specimens were initially reactive by the RPR, and 113 of these were reactive by MHA-TP. When using the EIA-RPR protocol, 170 specimens were initially positive by EIA, and of these, 112 were RPR reactive, indicating active disease. When compared to the routine protocol, the EIA-RPR protocol had sensitivity, specificity, and positive and negative predictive values of 96.5, 99.7, 97.3, and 99.7%, respectively. After resolution of discrepancies by additional testing, the adjusted sensitivity, specificity, and positive and negative predictive values were 100, 99.8, 98.3, and 100%, respectively. This evaluation demonstrates that when used in conjunction with the RPR, the Captia Syphilis EIA is a reliable method by which to test for syphilis.
对Captia梅毒IgG酶免疫测定法（EIA）与快速血浆反应素试验（RPR）联合使用作为梅毒检测方法进行了评估。按照RPR的常规实验室方案对总共1288份血清标本进行检测，随后对RPR反应性血清进行梅毒螺旋体微量血凝试验（MHA-TP）检测，以及采用EIA-RPR方案，即对EIA阳性标本先进行自动化EIA检测，然后进行手工RPR检测。采用常规方案时，最初有131份标本RPR呈反应性，其中113份MHA-TP呈反应性。采用EIA-RPR方案时，最初有170份标本EIA呈阳性，其中112份RPR呈反应性，表明存在活动性疾病。与常规方案相比，EIA-RPR方案的敏感性、特异性以及阳性和阴性预测值分别为96.5%、99.7%、97.3%和99.7%。通过额外检测解决差异后，调整后的敏感性、特异性以及阳性和阴性预测值分别为100%、99.8%、98.3%和100%。该评估表明，Captia梅毒EIA与RPR联合使用时，是一种可靠的梅毒检测方法。
