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本文引用的文献

1
Pseudo-outbreak of Enterococcus durans infections and colonization associated with introduction of an automated identification system software update.与引入自动鉴定系统软件更新相关的耐久肠球菌感染和定植的假性暴发。
J Clin Microbiol. 1996 Nov;34(11):2685-7. doi: 10.1128/jcm.34.11.2685-2687.1996.
2
Identification of Enterococcus faecalis strains by DNA hybridization and pulsed-field gel electrophoresis.通过DNA杂交和脉冲场凝胶电泳鉴定粪肠球菌菌株
J Clin Microbiol. 1995 Dec;33(12):3368-9. doi: 10.1128/jcm.33.12.3368-3369.1995.
3
Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. medical centers.对来自美国12家医疗中心的多重耐药粪肠球菌的评估与特性分析。
J Clin Microbiol. 1994 Nov;32(11):2840-2. doi: 10.1128/jcm.32.11.2840-2842.1994.
4
Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.通过聚合酶链反应(PCR)检测糖肽类耐药基因型并鉴定临床相关肠球菌的种属水平。
J Clin Microbiol. 1995 Jan;33(1):24-7. doi: 10.1128/jcm.33.1.24-27.1995.
5
DNA hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level.用于将肠球菌鉴定到种水平的DNA杂交和轮廓夹钳均匀电场电泳
J Clin Microbiol. 1995 Jan;33(1):141-5. doi: 10.1128/jcm.33.1.141-145.1995.
6
Ability of commercial and reference antimicrobial susceptibility testing methods to detect vancomycin resistance in enterococci.商业及参考抗菌药物敏感性检测方法检测肠球菌中万古霉素耐药性的能力。
J Clin Microbiol. 1995 Jun;33(6):1524-7. doi: 10.1128/jcm.33.6.1524-1527.1995.
7
Correlation between phenotypic characteristics and DNA relatedness within Enterococcus faecium strains.粪肠球菌菌株表型特征与DNA相关性之间的关系
J Clin Microbiol. 1995 Jun;33(6):1520-3. doi: 10.1128/jcm.33.6.1520-1523.1995.
8
Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR.通过荧光团增强重复序列PCR对耐甲氧西林金黄色葡萄球菌进行分子基因分型
J Clin Microbiol. 1995 Aug;33(8):2141-4. doi: 10.1128/jcm.33.8.2141-2144.1995.
9
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
10
Whole-cell protein patterns of nonhemolytic group B, type Ib, streptococci isolated from humans, mice, cattle, frogs, and fish.从人类、小鼠、牛、青蛙和鱼类中分离出的非溶血性B群Ib型链球菌的全细胞蛋白模式。
J Clin Microbiol. 1990 Mar;28(3):628-30. doi: 10.1128/jcm.28.3.628-630.1990.

一种用于鉴定屎肠球菌的聚合酶链反应检测法。

A PCR assay for identification of Enterococcus faecium.

作者信息

Cheng S, McCleskey F K, Gress M J, Petroziello J M, Liu R, Namdari H, Beninga K, Salmen A, DelVecchio V G

机构信息

Institute of Molecular Biology and Medicine, University of Scranton, Pennsylvania 18510, USA.

出版信息

J Clin Microbiol. 1997 May;35(5):1248-50. doi: 10.1128/jcm.35.5.1248-1250.1997.

DOI:10.1128/jcm.35.5.1248-1250.1997
PMID:9114416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC232738/
Abstract

Enterococcus faecium has recently emerged as a serious nosocomial pathogen. The prevalence and severity of enterococcal infections, the mortality rate from such infections, and the antibiotic resistance of enterococci are often species dependent. Since conventional biochemical methods fail to differentiate E. faecium from certain newly described enterococcal species, a PCR-based assay was developed for the rapid identification of E. faecium.

摘要

粪肠球菌最近已成为一种严重的医院病原体。肠球菌感染的发生率和严重程度、此类感染的死亡率以及肠球菌的抗生素耐药性通常取决于菌种。由于传统生化方法无法区分粪肠球菌与某些新描述的肠球菌菌种,因此开发了一种基于PCR的检测方法用于快速鉴定粪肠球菌。