通过聚合酶链反应（PCR）检测糖肽类耐药基因型并鉴定临床相关肠球菌的种属水平。
Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.
作者信息
Dutka-Malen S, Evers S, Courvalin P
机构信息
Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
出版信息
J Clin Microbiol. 1995 Jan;33(1):24-7. doi: 10.1128/jcm.33.1.24-27.1995.
Abstract
A PCR assay that allows simultaneous detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci (Enterococcus faecium, E. faecalis, E. gallinarum, and E. casseliflavus) was developed. This assay was based on specific amplification of internal fragments of genes encoding D-alanine:D-alanine ligases and related glycopeptide resistance proteins. The specificity of the assay was tested on 5 well-characterized glycopeptide-resistant strains and on 15 susceptible enterococcal type strains. Clinical isolates of enterococci that could not be identified to the species level by conventional methods were identified by the PCR test. This assay offers a specific and rapid alternative to antibiotic susceptibility tests, in particular for detection of low-level vancomycin resistance.
摘要
开发了一种聚合酶链反应（PCR）检测方法，可同时检测糖肽耐药基因型，并将临床相关肠球菌（粪肠球菌、屎肠球菌、鹑鸡肠球菌和格氏肠球菌）鉴定到种水平。该检测方法基于对编码D-丙氨酸：D-丙氨酸连接酶及相关糖肽耐药蛋白的基因内部片段进行特异性扩增。在5株特征明确的糖肽耐药菌株和15株敏感肠球菌标准菌株上测试了该检测方法的特异性。通过PCR检测对常规方法无法鉴定到种水平的临床肠球菌分离株进行了鉴定。该检测方法为抗生素敏感性试验提供了一种特异性和快速的替代方法，特别是用于检测低水平万古霉素耐药性。
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The vanB gene confers various levels of self-transferable resistance to vancomycin in enterococci.vanB基因赋予肠球菌对万古霉素不同水平的自身可转移性耐药性。
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