Delamarche E, Bernard A, Schmid H, Michel B, Biebuyck H
IBM Research Division, Zurich Research Laboratory, CH-8803 Rüschlikon, Switzerland.
Science. 1997 May 2;276(5313):779-81. doi: 10.1126/science.276.5313.779.
Microfluidic networks (microFNs) were used to pattern biomolecules with high resolution on a variety of substrates (gold, glass, or polystyrene). Elastomeric microFNs localized chemical reactions between the biomolecules and the surface, requiring only microliters of reagent to cover square millimeter-sized areas. The networks were designed to ensure stability and filling of the microFN and allowed a homogeneous distribution and robust attachment of material to the substrate along the conduits in the microFN. Immunoglobulins patterned on substrates by means of microFNs remained strictly confined to areas enclosed by the network with submicron resolution and were viable for subsequent use in assays. The approach is simple and general enough to suggest a practical way to incorporate biological material on technological substrates.
微流体网络（microFNs）被用于在各种底物（金、玻璃或聚苯乙烯）上高分辨率地对生物分子进行图案化。弹性微流体网络使生物分子与表面之间的化学反应局部化，仅需几微升试剂就能覆盖平方毫米大小的区域。这些网络的设计确保了微流体网络的稳定性和填充，并允许材料沿着微流体网络中的管道在底物上均匀分布且牢固附着。通过微流体网络在底物上图案化的免疫球蛋白严格局限于网络所包围的区域，分辨率可达亚微米级别，并且在后续检测中仍可使用。该方法简单且通用，为在技术底物上整合生物材料提供了一种实用途径。
