Davies S, Bain B J
Department of Haematology, St Mary's Hospital Medical School, London, UK.
Clin Lab Haematol. 1996 Mar;18(1):35-8. doi: 10.1111/j.1365-2257.1996.tb00734.x.
The precision, accuracy, stability and clinical usefulness of the basophil count of a Technicon H1 automated blood cell counter were assessed and a reference range for the absolute basophil count was derived. As expected, the precision was considerably better than that of a manual count but it was worse than would have been expected if the only inaccuracy were the random error consequent on the number of cells counted. Accuracy was sufficient for the count to be of some clinical use but an elevated 'basophil' count was more often consequent on 'pseudo-basophilia' than on true basophilia. As a consequence it is necessary to confirm elevated counts by microscopic examination of a blood film whenever the presence of an elevated count is diagnostically important. The basophil count was stable for up to 3 days, both at room temperature and at 4 degrees C. The reference range was 0.02-0.09 x 10(9)/I. An elevated 'basophil' count serves to alert the instrument operator to the presence of an abnormal blood sample. Since the technology used to count basophils is the same in H1, H2 and H3 instruments, our results can be generalized to other instruments in the series.
对Technicon H1自动血细胞计数器的嗜碱性粒细胞计数的精密度、准确度、稳定性及临床实用性进行了评估，并得出了绝对嗜碱性粒细胞计数的参考范围。正如预期的那样，其精密度比手工计数要好得多，但如果唯一的不准确度是因所计数细胞数量产生的随机误差，那么它比预期的要差。准确度足以使该计数在一定程度上具有临床用途，但“嗜碱性粒细胞”计数升高更多是由“假性嗜碱性粒细胞增多”而非真正的嗜碱性粒细胞增多导致的。因此，每当计数升高对诊断具有重要意义时，有必要通过血液涂片显微镜检查来确认升高的计数。嗜碱性粒细胞计数在室温及4摄氏度下均可稳定长达3天。参考范围为0.02 - 0.09×10⁹/I。“嗜碱性粒细胞”计数升高有助于提醒仪器操作人员存在异常血样。由于H1、H2和H3仪器用于计数嗜碱性粒细胞的技术相同，我们的结果可推广至该系列的其他仪器。
