Wilson J D, Cariello N F
Medicine Safety Evaluation, Glaxo Wellcome Inc., Research Triangle Park, North Carolina 27709, USA.
Environ Mol Mutagen. 1997;29(2):217-9. doi: 10.1002/(sici)1098-2280(1997)29:2<217::aid-em12>3.0.co;2-h.
Sodium azide, when added to wells adjacent to untreated wells, caused an increase in the reversion rate of Salmonella typhimurium TA100 in a 12-well plate format. Increases in the reversion frequency in adjacent, untreated wells were observed when a single well on the plate was treated with as little as 1 microg of sodium azide. This effect is probably caused by the hydrolysis of sodium azide to form hydrazoic acid. Hydrazoic acid has a boiling point of 37 degrees C and, thus, would become a diffusible gas during the incubation of the plates. Our findings suggest that a diffusible gas is present and that this gas has the ability to contaminate nearby wells when using the multiwell version of the Ames assay. Furthermore, it may be prudent to isolate all positive controls and negative controls on separate plates with no test material since a volatile test material could produce spurious results in the Ames miniscreen.
在12孔板中，将叠氮化钠添加到与未处理孔相邻的孔中时，会导致鼠伤寒沙门氏菌TA100回复突变率增加。当平板上的单个孔用低至1微克的叠氮化钠处理时，可观察到相邻未处理孔的回复突变频率增加。这种效应可能是由于叠氮化钠水解形成叠氮酸所致。叠氮酸的沸点为37℃，因此在平板培养期间会变成可扩散的气体。我们的研究结果表明，存在一种可扩散的气体，并且在使用Ames试验的多孔版本时，这种气体有能力污染附近的孔。此外，由于挥发性测试材料可能在Ames微型筛选中产生假结果，因此将所有阳性对照和阴性对照单独置于没有测试材料的平板上可能是谨慎的做法。
