Rahlfs S, Müller V
Institut für Mikrobiologie der Universität, Göttingen, Germany.
FEBS Lett. 1997 Mar 10;404(2-3):269-71. doi: 10.1016/s0014-5793(97)00088-4.
A 3.2 kb EcoRI fragment carrying genes for Na(+)-F1F0 ATPase was cloned from chromosomal DNA of Acetobacterium woodii. DNA sequence analysis revealed the presence of an open reading frame which was identified by data base searches and comparison with the experimentally derived N-terminal amino acid sequence to code for subunit c of Na(+)-F1F0 ATPase. A comparison of the primary sequences of the two well established Na(+)-translocating F1F0 ATPases from Acetobacterium woodii and Propionigenium modestum with H(+)-translocating enzymes indicates the length of the C-terminus as well as specific residues located in the cytoplasmic membrane to be important for Na+ transport.
从伍氏醋杆菌的染色体DNA中克隆出一个携带Na(+)-F1F0 ATP酶基因的3.2 kb EcoRI片段。DNA序列分析显示存在一个开放阅读框，通过数据库搜索并与实验获得的N端氨基酸序列进行比较，确定该开放阅读框编码Na(+)-F1F0 ATP酶的c亚基。将伍氏醋杆菌和适度丙酸杆菌这两种已明确的Na(+)-转运F1F0 ATP酶的一级序列与H(+)-转运酶进行比较，结果表明C端的长度以及位于细胞质膜中的特定残基对于Na+转运很重要。
