Heise R, Müller V, Gottschalk G
Institut für Mikrobiologie, Göttingen, Federal Republic of Germany.
Eur J Biochem. 1992 Jun 1;206(2):553-7. doi: 10.1111/j.1432-1033.1992.tb16959.x.
Inverted membrane vesicles of the homoacetogenic bacterium Acetobacterium woodii catalyzed the hydrolysis of ATP with a rate of 100-150 nmol.min-1.mg protein-1. The ATPase was stimulated 1.4-1.6-fold by NaCl and inhibited by N,N'-dicyclohexylcarbodiimide tributyltin or azide. The degree of inhibition caused by F0-directed but not F1-directed inhibitors was affected by the Na+ concentration in the medium. These experiments indicated the presence of a sodium-translocating ATPase. This was verified by transport studies. Upon addition of ATP to inverted vesicles, 22Na+ was actively transported into the intravesicular space up to a 24-fold accumulation. Na+ transport was inhibited by the sodium ionophore N,N,N',N',-tetracyclohexyl-1,2-phenyl-enedioxydiacetamide but stimulated by valinomycin with potassium whereas the protonophore 3,5,-di-tert-butyl-4-hydroxybenzylidenemalonitrile was without effect. N,N'-dicyclohexylcarbodiimide and tributyltin inhibited 22Na+ transport. These experiments are in accordance with a primary electrogenic Na+ transport as catalyzed by a F1F0-ATPase.
同型产乙酸菌伍氏醋酸杆菌的内翻膜囊泡催化ATP水解的速率为100 - 150 nmol·min⁻¹·mg蛋白⁻¹。ATP酶受到NaCl的刺激，活性提高1.4 - 1.6倍，并受到N,N'-二环己基碳二亚胺、三丁基锡或叠氮化物的抑制。由F0导向而非F1导向的抑制剂所引起的抑制程度受培养基中Na⁺浓度的影响。这些实验表明存在一种钠转运ATP酶。这通过转运研究得到了验证。向内翻囊泡中添加ATP后，²²Na⁺被主动转运到囊泡内空间，积累倍数高达24倍。Na⁺转运受到钠离子载体N,N,N',N',-四环己基-1,2-苯二氧基二乙酰胺的抑制，但缬氨霉素与钾离子共同作用时会刺激其转运，而质子载体3,5,-二叔丁基-4-羟基苄叉丙二腈则无影响。N,N'-二环己基碳二亚胺和三丁基锡抑制²²Na⁺转运。这些实验与F1F0 - ATP酶催化的初级电致Na⁺转运一致。
