Ginocchio C C, Rahn K, Clarke R C, Galán J E
Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794-5222, USA.
Infect Immun. 1997 Apr;65(4):1267-72. doi: 10.1128/iai.65.4.1267-1272.1997.
We have identified several environmental isolates of Salmonella senftenberg and S. litchfield which carry a deletion encompassing a vast segment of the centisome 63 region of the Salmonella chromosome. The deletion includes the entire inv, spa, and hil loci, which are required for entry of Salmonella spp. into mammalian cells. Consequently, these isolates were found to be markedly deficient in the ability to enter cultured epithelial cells. In contrast, no deletions were found in the corresponding regions of the chromosomes of clinical isolates of these serovars; consequently, these isolates were found to be highly invasive for cultured epithelial cells. These data confirm the importance of the centisome 63 region of the Salmonella chromosome in mediating the entry of these organisms into cultured mammalian cells and indicate that additional entry pathways are presumably not utilized by these environmental isolates. These results are also consistent with the notion that this region constitutes a pathogenicity island which remains unstable in certain Salmonella serotypes.
我们已经鉴定出几种肠炎沙门氏菌和利奇菲尔德沙门氏菌的环境分离株，它们携带一个缺失片段，该片段涵盖了沙门氏菌染色体63分摩区域的很大一部分。该缺失包括沙门氏菌属进入哺乳动物细胞所需的整个inv、spa和hil基因座。因此，发现这些分离株进入培养的上皮细胞的能力明显不足。相比之下，在这些血清型临床分离株染色体的相应区域未发现缺失；因此，发现这些分离株对培养的上皮细胞具有高度侵袭性。这些数据证实了沙门氏菌染色体63分摩区域在介导这些微生物进入培养的哺乳动物细胞中的重要性，并表明这些环境分离株可能未利用其他进入途径。这些结果也与该区域构成一个致病岛的观点一致，该致病岛在某些沙门氏菌血清型中仍然不稳定。
