Jafri S I
Stain Technol. 1979 Mar;54(2):93-5. doi: 10.3109/10520297909112641.
A new combined stain is described for the study of cell types in the fish pituitary. Tissues are prepared by fixing in formol-sublimate and then embedded in paraffin wax. Tissue is sectioned at 5 micron and than stained sequentially with performic acid-alcian blue, periodic acid-Schiff, orange G, and acid fuchsin. As a result of this procedure acidophils stain as follows: lactotropes, red; corticotropes, light pink; melanotropes, bright pink; and somatotropes, orange. Cyanophils stain either magenta red (gonadotropes) or blue (thyrotropes). Neurosecretory material and the fibers of the pars nervosa which penetrate the pars intermedia stain light blue.
描述了一种用于研究鱼类垂体细胞类型的新型复合染色法。组织经甲醛 - 升汞固定后，再嵌入石蜡。将组织切成5微米厚的切片，然后依次用过甲酸 - 阿尔辛蓝、高碘酸 - 席夫试剂、橘黄G和酸性品红染色。经过该程序后，嗜酸性细胞的染色情况如下：催乳细胞呈红色；促肾上腺皮质激素细胞呈浅粉色；促黑素细胞呈亮粉色；生长激素细胞呈橙色。嗜碱性细胞要么染成品红色（促性腺激素细胞），要么染成蓝色（促甲状腺激素细胞）。神经分泌物质以及穿过中间部的神经部纤维染成浅蓝色。
