Kandelaki N E, Chanishvili T G, Moshiashvili I Ia
Zh Mikrobiol Epidemiol Immunobiol. 1979 Aug(8):26-31.
For the first time O antigens obtained from phagolysates were proved to be suitable for use as material for the production of highly specific erythrocyte diagnostic preparations. O antigens obtained from Salmonella by two methods, i.e. phage disintegration and Grasset's method, were subjected to comparative chemical analysis and found to have no essential difference. Nevertheless, the sensitizing potency of O antigens obtained from phagolysates were experimentally shown to be 3 times greater than that of O antigens obtained by Grasset's method. The optimum sensitizing doses established in the passive hemagglutination test for O antigens obtained by both methods indicated that these antigens were highly sensitive and specific.
首次证明，从吞噬溶酶体中获得的O抗原适合用作生产高特异性红细胞诊断制剂的材料。通过两种方法（即噬菌体裂解和格拉塞氏法）从沙门氏菌中获得的O抗原进行了比较化学分析，发现没有本质区别。然而，实验表明，从吞噬溶酶体中获得的O抗原的致敏效力比通过格拉塞氏法获得的O抗原高3倍。两种方法获得的O抗原在被动血凝试验中确定的最佳致敏剂量表明，这些抗原具有高度的敏感性和特异性。
