Grandadam M, Dupin N, Calvez V, Gorin I, Blum L, Kernbaum S, Sicard D, Buisson Y, Agut H, Escande J P, Huraux J M
Laboratoire de Virologie, CNRS EP 57, Hôpital Tarnier, Paris, France.
J Infect Dis. 1997 May;175(5):1198-201. doi: 10.1086/593567.
The epidemiologic link between multicentric Castleman's disease (MCD) and Kaposi's sarcoma (KS) and the high frequency of KS herpesvirus (KSHV) detection in both diseases raise the question of a role of this new virus in the pathogenesis of MCD. To explore this hypothesis, the KSHV DNA load was investigated in peripheral blood mononuclear cells of 3 human immunodeficiency virus (HIV)-infected patients with MCD at different points during the clinical course. Clinical parameters, such as fever and the presence of lymphadenopathy, were systematically assessed. Hemogram and C-reactive protein level determinations were performed as standard procedures. KSHV DNA load was investigated by means of semiquantitative polymerase chain reaction assay using peripheral blood mononuclear cells of the patients. A correlation between the variation in clinical and biologic parameters related to MCD and KSHV DNA load was found, suggesting a close relationship between KSHV and MCD in HIV-1-infected patients.
多中心Castleman病（MCD）与卡波西肉瘤（KS）之间的流行病学关联以及在这两种疾病中检测到卡波西肉瘤疱疹病毒（KSHV）的高频率，引发了这种新病毒在MCD发病机制中作用的问题。为了探讨这一假设，在临床过程中的不同时间点，对3例感染人类免疫缺陷病毒（HIV）且患有MCD的患者外周血单个核细胞中的KSHV DNA载量进行了研究。系统评估了发热和淋巴结病等临床参数。按照标准程序进行血常规和C反应蛋白水平测定。使用患者的外周血单个核细胞，通过半定量聚合酶链反应分析来研究KSHV DNA载量。发现与MCD相关的临床和生物学参数变化与KSHV DNA载量之间存在相关性，这表明在HIV-1感染患者中，KSHV与MCD之间存在密切关系。
