Colberg-Poley A M
Center for Virology, Immunology and Infectious Disease Research, Children's National Medical Center, Washington, D.C. 20010, USA.
Intervirology. 1996;39(5-6):350-60. doi: 10.1159/000150506.
Human cytomegalovirus (HCMV) encodes multiple regulatory proteins at immediate early (IE) times of infection. Ancillary IE proteins are encoded by the UL36-38, UL115-119, TRS1/IRS1 and US3 loci. In contrast to the major IE nuclear proteins, several of the ancillary IE proteins are type I integral membrane N-glycoproteins. Nonetheless, all of the ancillary proteins examined to date have the ability to regulate nuclear gene expression and to interact cooperatively. Significantly, products from the UL36-38 and TRS1/IRSI IE loci as well as products from the MIE locus are required for HCMV ori-Lyt DNA replication. Moreover, the products of the UL36 and UL37 IE genes are essential for HCMV growth in human cells. Finally, one ancillary IE glycoprotein, gpUS3, is known to have a nonregulatory function; that is, gpUS3 binds and retains major histocompatibility complex class I heavy chains in the endoplasmic reticulum, thereby inhibiting antigen presentation. Thus, the functional presence of multiple IE proteins is required during HCMV replication both in vitro and in vivo to orchestrate necessary events for HCMV replication as well as for the survival of the infected host cell.
人巨细胞病毒（HCMV）在感染的即刻早期（IE）阶段编码多种调节蛋白。辅助IE蛋白由UL36 - 38、UL115 - 119、TRS1/IRS1和US3基因座编码。与主要的IE核蛋白不同，几种辅助IE蛋白是I型整合膜N - 糖蛋白。尽管如此，迄今为止检测的所有辅助蛋白都具有调节核基因表达和协同相互作用的能力。值得注意的是，HCMV ori - Lyt DNA复制需要来自UL36 - 38和TRS1/IRSI IE基因座的产物以及来自MIE基因座的产物。此外，UL36和UL37 IE基因的产物对于HCMV在人细胞中的生长至关重要。最后，一种辅助IE糖蛋白gpUS3已知具有非调节功能；也就是说，gpUS3在内质网中结合并保留主要组织相容性复合体I类重链，从而抑制抗原呈递。因此，在HCMV体外和体内复制过程中，需要多种IE蛋白的功能性存在来协调HCMV复制以及被感染宿主细胞存活所需的必要事件。
