Houshmand M, Holme E, Hanson C, Wennerholm U B, Hamberger L
Department of Clinical Chemistry, Göteborg University, Sahlgren's Hospital, Gothenburg, Sweden.
J Assist Reprod Genet. 1997 Apr;14(4):223-7. doi: 10.1007/BF02766114.
During intracytoplasmic sperm injection (ICSI) the whole sperm, including head, midpiece and tail, is injected into the middle area of the oocyte. To find out what happens to the sperm mitochondria after ICSI, we checked the first six children born after ICSI treatment for occurrence of paternal mitochondrial DNA (mtDNA). The difference between maternal and paternal mtDNA in the investigated couples in our study was confined to single-base pair substitutions and we had to rely on restriction enzyme cleavage to differentiate between the mitochondrial genomes of the parents. With this kind of assay we were able to reach a sensitivity of about 0.2% for the paternal mtDNA. However, as uneven partition between tissues of heteroplasmic mtDNA is expected to occur, it would not be unlikely that an enrichment to 0.2% would occur in a given tissue if paternal mtDNA was transmitted by the ICSI procedure. We did not detect this level in the blood in any of the six children.
在卵胞浆内单精子注射（ICSI）过程中，整个精子，包括头部、中段和尾部，被注入到卵母细胞的中间区域。为了弄清楚ICSI后精子线粒体的变化情况，我们检查了ICSI治疗后出生的头六个孩子，以检测父系线粒体DNA（mtDNA）的存在情况。在我们研究的受试夫妇中，母系和父系mtDNA的差异仅限于单碱基对替换，我们不得不依靠限制性酶切来区分父母的线粒体基因组。通过这种检测方法，我们对父系mtDNA的检测灵敏度约为0.2%。然而，由于预计异质性mtDNA在组织间会出现不均匀分配，如果父系mtDNA通过ICSI程序传递，那么在特定组织中富集到0.2%并非不可能。我们在这六个孩子的血液中均未检测到这一水平。
