Sengoku K, Tamate K, Takuma N, Yoshida T, Goishi K, Ishikawa M
Department of Obstetrics and Gynecology, Asahikawa Medical College, Nishikagura, Japan.
Hum Reprod. 1997 Mar;12(3):474-7. doi: 10.1093/humrep/12.3.474.
The present study was designed to compare the cycle characteristics of in-vitro fertilization (IVF) and the chromosomal normality of oocytes in patients with polycystic ovarian syndrome (PCOS) with those of patients with tubal factor infertility. In all, 28 cycles of 24 PCOS patients and 55 cycles of 31 patients with tubal factor infertility (control) were investigated. Although a significantly greater number of oocytes were retrieved from PCOS patients (mean +/- SD: 15.6 +/- 6.4 versus 9.0 +/- 4.0, PCOS versus control group, P < 0.05), the percentage of fertilized oocytes was significantly lower in the PCOS group compared with controls (40.1 versus 73.8%, P < 0.01). The pregnancy rate per embryo transfer did not differ between the two groups. Cytogenetic analysis was performed on 74 oocytes from PCOS patients and 73 oocytes from control patients. In the PCOS group, 10 of the 74 oocytes (13.5%) demonstrated aneuploidy, four (5.4%) oocytes were diploid and six (8.1%) oocytes were metaphase II with a prematurely condensed sperm chromosome (PCC). In the tubal infertility group, nine of the 73 (12.3%) oocytes showed aneuploidy, four (5.5%) oocytes were diploid and five (6.8%) oocytes were found to have PCC. There was no significant difference in the aneuploidy, diploidy and PCC rates between the two groups. These results suggest that the reduced fertilization observed in PCOS is not attributable to chromosomal aberrations or immaturity of oocytes recruited from patients with PCOS.
本研究旨在比较多囊卵巢综合征（PCOS）患者与输卵管因素不孕症患者的体外受精（IVF）周期特征及卵母细胞的染色体正常性。共调查了24例PCOS患者的28个周期以及31例输卵管因素不孕症患者（对照组）的55个周期。尽管从PCOS患者中获取的卵母细胞数量显著更多（平均值±标准差：15.6±6.4对9.0±4.0，PCOS组对对照组，P<0.05），但与对照组相比，PCOS组的受精卵母细胞百分比显著更低（40.1%对73.8%，P<0.01）。两组间每次胚胎移植的妊娠率无差异。对PCOS患者的74个卵母细胞和对照组患者的73个卵母细胞进行了细胞遗传学分析。在PCOS组中，74个卵母细胞中有10个（13.5%）表现为非整倍体，4个（5.4%）卵母细胞为二倍体，6个（8.1%）卵母细胞为中期II且带有过早凝聚的精子染色体（PCC）。在输卵管不孕症组中，73个卵母细胞中有9个（12.3%）显示为非整倍体，4个（5.5%）卵母细胞为二倍体，5个（6.8%）卵母细胞发现有PCC。两组间非整倍体、二倍体和PCC率无显著差异。这些结果表明，PCOS中观察到的受精率降低并非归因于PCOS患者募集的卵母细胞的染色体畸变或不成熟。
