糖尿病性白内障中的晶状体脂质过氧化物和谷胱甘肽浓度

Lens lipid peroxides and glutathione concentrations in diabetic cataract.

作者信息

Ozmen D, Mutaf I, Ozmen B, Mentes J, Bayindir O

机构信息

Department of Biochemistry, Ege University Medical School, Izmir, Turkey.

出版信息

Ann Clin Biochem. 1997 Mar;34 ( Pt 2):190-2. doi: 10.1177/000456329703400211.

DOI:10.1177/000456329703400211

PMID:9133255

Abstract

This study aims to explore the role of reactive oxygen radicals in the genesis of diabetic cataract. Lipid peroxide (LPO) concentrations in senile (n = 30) and diabetic (n = 14) cataractous lenses, were determined as thiobarbituric acid-reactive substances (TBARS) by a method modified from Satoh and Yagi, and reduced glutathione (GSH) concentrations were measured according to Beutler. Lens LPO levels (mean, SD; nmol TBARS/g protein) were significantly higher in diabetics (107.54, 18.12) than senile cataractous subjects (53.54, 15.48) (P < 0.0001). Lens GSH levels (mean, SD; nmol/g protein) showed no significant difference between diabetics (4.29, 2.05) and senile cataractous subjects (4.68, 3.12). These results suggest that free radical damage is more effective in the genesis of diabetic cataract than in senile cataract.

摘要

本研究旨在探讨活性氧自由基在糖尿病性白内障发生中的作用。采用改良自Satoh和Yagi的方法，将老年（n = 30）和糖尿病（n = 14）白内障晶状体中的脂质过氧化物（LPO）浓度测定为硫代巴比妥酸反应性物质（TBARS），并根据Beutler法测量还原型谷胱甘肽（GSH）浓度。糖尿病患者晶状体LPO水平（均值，标准差；nmol TBARS/g蛋白）为（107.54，18.12），显著高于老年白内障患者（53.54，15.48）（P < 0.0001）。糖尿病患者晶状体GSH水平（均值，标准差；nmol/g蛋白）为（4.29，2.05），与老年白内障患者（4.68，3.12）相比无显著差异。这些结果表明，自由基损伤在糖尿病性白内障发生中比在老年性白内障中更有效。