Marder M L, Channing C P, Schwartz N B
Endocrinology. 1977 Nov;101(5):1939-42. doi: 10.1210/endo-101-5-1639.
Sham ovariectomy (SOV) or ovariectomy (OVX) was performed at 0800h of metastrus; porcine serum (PS) or porcine follicular fluid (PFF) was injected intraperitoneally at 0830h and/or 1130h, with terminal blood samples collected at 1700h. Fluid from medium and large follicles in porcine ovaries, charcoal extracted to remove steroids, significantly reduced serum FSH, in a dose-dependent manner. Futhermore, the SOV rats were more sensitive to PFF with respect to serum FSH lowering than the OVX rats, although when enough PFF was injected, both groups showed the same level of suppression. Since serum LH was very low, the possible effect of PFF on this hormone could not be assessed.
在发情后期的08:00进行假卵巢切除术（SOV）或卵巢切除术（OVX）；在08:30和/或11:30腹腔注射猪血清（PS）或猪卵泡液（PFF），并在17:00采集终末血样。从猪卵巢的中、大卵泡中提取的液体经活性炭处理以去除类固醇，能以剂量依赖的方式显著降低血清促卵泡激素（FSH）。此外，在降低血清FSH方面，SOV大鼠比OVX大鼠对PFF更敏感，不过当注射足够量的PFF时，两组的抑制水平相同。由于血清促黄体生成素（LH）非常低，无法评估PFF对该激素的可能影响。
