Rolland L, Zilberfarb V, Furtado A, Gentilini M
Laboratoire de Parasitologie, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Parasite Immunol. 1994 Nov;16(11):599-608. doi: 10.1111/j.1365-3024.1994.tb00316.x.
We have analysed by immunoblotting sera from humans and dogs with visceral leishmaniasis, from the Old World as well as the New. When lysates of promastigotes are used as antigens, antibodies against a 94 kDa Leishmania component are detected, regardless of the age and geographical origin of the patient, the serum antibody titre as measured by indirect immunofluorescence, and the number of arcs in counterimmunoelectrophoresis. Low dilutions of sera from patients with Old and New World cutaneous leishmaniasis did not react with the 94-kDa antigen, whatever the species of Leishmania used as antigens. Sera from patients with other infections than leishmaniases, or without infection, are negative, even at low dilution. Anti-94 kDa antibodies were detected in the sera of Leishmania-infected dogs from both the Old and the New World. When lysates of Leishmania mexicana axenic amastigotes are used as antigens, the 94-kDa antigen was little or none identified by sera from humans and dogs with visceral leishmaniasis, and never recognized by control sera. Thus, the specific recognition of the 94-kDa promastigote antigen in human and canine visceral leishmaniasis suggests that this antigen could be a potential candidate in the differential immunodiagnosis of the disease.
我们通过免疫印迹法分析了来自新旧世界患内脏利什曼病的人类和犬类的血清。当使用前鞭毛体裂解物作为抗原时，无论患者的年龄和地理来源、通过间接免疫荧光法测得的血清抗体滴度以及对流免疫电泳中的沉淀弧线数量如何，均可检测到针对一种94 kDa利什曼原虫成分的抗体。新旧世界皮肤利什曼病患者的血清低稀释度样本，无论用作抗原的利什曼原虫种类如何，均不与94 kDa抗原发生反应。患有除利什曼病以外的其他感染或未感染的患者血清，即使在低稀释度下也呈阴性。在新旧世界感染利什曼原虫的犬类血清中均检测到了抗94 kDa抗体。当使用墨西哥利什曼原虫无细胞型无鞭毛体裂解物作为抗原时，患内脏利什曼病的人类和犬类血清几乎未识别或未识别出94 kDa抗原，对照血清也从未识别过该抗原。因此，人类和犬类内脏利什曼病中对94 kDa前鞭毛体抗原的特异性识别表明，该抗原可能是该病鉴别免疫诊断的潜在候选物。
