Mattern K A, van Goethem R E, de Jong L, van Driel R
E.C. Slater Instituut, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
J Cell Biochem. 1997 Apr;65(1):42-52. doi: 10.1002/(sici)1097-4644(199704)65:1<42::aid-jcb5>3.0.co;2-z.
The nuclear matrix may be involved in the structural and functional organization of the cell nucleus. However, we still do not understand the molecular basis of the intranuclear fibrogranular network that is part of the nuclear matrix. We recently described a method to identify internal nuclear matrix proteins [Mattern et al. (1996): J Cell Biochem 62:275-289], which was done by comparing two nuclear matrix preparations: one with and one without the internal structure by using quantitative two-dimensional gel electrophoresis. In the present study, we use the same approach to compare the nuclear matrix proteins of four different human cell types to investigate whether they have a similar internal nuclear matrix protein composition. Major nuclear matrix proteins present in all these cell types likely represent the base of the internal nuclear matrix. We demonstrate that the 25 most abundant internal nuclear matrix proteins are common to all four cell types. Together, these common proteins represent more than 75% of the total internal nuclear matrix protein mass in each cell type. This set of proteins includes B23 and most hnRNP proteins. The quantity of most of these proteins is very similar in the four cell types. The fact that the internal nuclear matrix consists mainly of hnRNP proteins, which may be involved in transcription, transport, and processing of hnRNA, supports the idea that the internal nuclear matrix is the result of these processes.
核基质可能参与细胞核的结构和功能组织。然而，我们仍然不了解作为核基质一部分的核内纤维颗粒网络的分子基础。我们最近描述了一种鉴定核内基质蛋白的方法[马特恩等人（1996年）：《细胞生物化学杂志》62:275 - 289]，该方法通过比较两种核基质制剂来完成：一种有内部结构，另一种没有内部结构，采用定量二维凝胶电泳。在本研究中，我们使用相同的方法比较四种不同人类细胞类型的核基质蛋白，以研究它们是否具有相似的核内基质蛋白组成。所有这些细胞类型中存在的主要核基质蛋白可能代表核内基质的基础。我们证明，所有四种细胞类型共有25种最丰富的核内基质蛋白。这些共同的蛋白一起占每种细胞类型核内基质蛋白总量的75%以上。这组蛋白包括B23和大多数hnRNP蛋白。这些蛋白中的大多数在四种细胞类型中的数量非常相似。核内基质主要由可能参与hnRNA转录、运输和加工的hnRNP蛋白组成，这一事实支持了核内基质是这些过程结果的观点。
