Zhang J C, Savage H E, Sacks P G, Delohery T, Alfano R R, Katz A, Schantz S P
Head and Neck Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Lasers Surg Med. 1997;20(3):319-31. doi: 10.1002/(sici)1096-9101(1997)20:3<319::aid-lsm11>3.0.co;2-8.
The objective of this study was to examine the question of whether unique spectral patterns were associated with cell proliferation and could be identified by comparing the fluorescence pattern of slow to rapid growing cells.
STUDY DESIGN/MATERIALS AND METHODS: Three in vitro model systems, (A431 cells inhibited by EGF, serum-starved 3T3 fibroblasts, and normal oral epithelial cells exposed to TGF beta), were analyzed using fluorescence spectroscopy. Growth status was monitored by cell number, 3H-thymidine incorporation, and flow cytometry.
The excitation spectra (lambda ex 240-430 nm, lambda em 450 nm) effectively distinguished slow and rapid growing cells in all three systems. Statistical analysis of the ratios of the main broad peak (320-350 nm) to a point on the down-slope of the curve at 370 nm was statistically significant. Ratios in the emission scan (lambda ex 340 nm, lambda em 360-660 nm) could separate slow and rapid growing A431 and oral epithelial cells (P = 0.0001 and P = 0.023, respectively), but not slow and fast growing 3T3 cells (P = 0.56).
Innate cellular fluorescence has the potential to discriminate proliferating and nonproliferating cell populations.
本研究的目的是探讨独特的光谱模式是否与细胞增殖相关，以及能否通过比较生长缓慢和快速的细胞的荧光模式来识别。
研究设计/材料与方法：使用荧光光谱法分析了三种体外模型系统（受表皮生长因子抑制的A431细胞、血清饥饿的3T3成纤维细胞以及暴露于转化生长因子β的正常口腔上皮细胞）。通过细胞数量、3H-胸腺嘧啶核苷掺入和流式细胞术监测生长状态。
激发光谱（激发波长240 - 430nm，发射波长450nm）在所有三种系统中均能有效区分生长缓慢和快速的细胞。对主峰（320 - 350nm）与曲线370nm处下坡点的比率进行统计分析，具有统计学意义。发射扫描光谱（激发波长340nm，发射波长360 - 660nm）中的比率能够区分生长缓慢和快速的A431细胞及口腔上皮细胞（P分别为0.0001和0.023），但不能区分生长缓慢和快速的3T3细胞（P = 0.56）。
天然细胞荧光有潜力区分增殖和非增殖细胞群体。
