Ohata T, Fukuda K, Takahashi M, Sugimura T, Wakabayashi K
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1997 Mar;88(3):234-7. doi: 10.1111/j.1349-7006.1997.tb00372.x.
Although nitric oxide (NO) is an important biological mediator, its excessive production in inflammation is thought to be a causative factor for cellular injury and, over the long term, cancer. In the present study, the effects of several fatty acids on NO production in murine macrophage cell line RAW264 cells stimulated with lipopolysaccharide were examined. Suppression of NO production was observed with the omega 3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid, eicosapentaenoic acid and alpha-linolenic acid, in a dose-dependent fashion. In contrast, no inhibition was observed with omega 6 PUFA (linoleic acid), omega 9 PUFA (oleic acid) or a saturated fatty acid (stearic acid). Western and northern blot analyses suggested that suppression of the induction of inducible NO synthase gene expression is responsible for the inhibition of NO production by omega 3 PUFAs. The inhibitory effect of omega 3 PUFA on NO production in activated macrophages could contribute to their cancer chemopreventive influence.
尽管一氧化氮（NO）是一种重要的生物介质，但在炎症过程中其过量产生被认为是导致细胞损伤的一个因素，长期来看还会引发癌症。在本研究中，检测了几种脂肪酸对用脂多糖刺激的小鼠巨噬细胞系RAW264细胞中NO产生的影响。观察到ω-3多不饱和脂肪酸（PUFA）二十二碳六烯酸、二十碳五烯酸和α-亚麻酸以剂量依赖的方式抑制NO的产生。相比之下，ω-6 PUFA（亚油酸）、ω-9 PUFA（油酸）或饱和脂肪酸（硬脂酸）未观察到抑制作用。蛋白质免疫印迹和Northern印迹分析表明，ω-3 PUFAs抑制NO产生的原因是抑制了诱导型NO合酶基因表达的诱导。ω-3 PUFA对活化巨噬细胞中NO产生的抑制作用可能有助于其癌症化学预防作用。
