紫外线照射的人类细胞中胸腺嘧啶二聚体切除的动力学
The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
作者信息
Ehmann U K, Cook K H, Friedberg E C
出版信息
Biophys J. 1978 May;22(2):249-64. doi: 10.1016/S0006-3495(78)85487-3.
Abstract
We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21-85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12-20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12-22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.
摘要
我们研究了几种经紫外线（UV）照射的培养人类细胞系酸不溶性部分中胸腺嘧啶二聚体损失的动力学。我们的结果表明，10至40 J/m2的紫外线通量平均每个细胞产生21 - 85×10⁵个胸腺嘧啶二聚体，每个细胞最终最大损失12 - 20×10⁵个胸腺嘧啶二聚体。二聚体损失一半所需的时间在紫外线照射后为12 - 22小时。相比之下，通过放射自显影测量的DNA半最大修复合成时间为4.5小时。这个数字与报道的半最大修复合成时间非常吻合，根据我们的分析，其范围为0.5至3.6小时。从体内胸腺嘧啶二聚体切除的可能分子机制以及可能的实验假象方面讨论了DNA中胸腺嘧啶二聚体损失动力学与修复合成之间的差异。
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