杆状病毒表达的蓝舌病毒非结构蛋白NS2可在小鼠体内诱导细胞毒性T细胞反应，提供部分保护。
Baculovirus-expressed nonstructural protein NS2 of bluetongue virus induces a cytotoxic T-cell response in mice which affords partial protection.
作者信息
Jones L D, Williams T, Bishop D, Roy P
机构信息
Institute of Virology & Environmental Microbiology, Oxford, United Kingdom.
出版信息
Clin Diagn Lab Immunol. 1997 May;4(3):297-301. doi: 10.1128/cdli.4.3.297-301.1997.
Abstract
Virus-specific cytotoxic T lymphocytes were generated in two strains of mice (BALB/c and CBA/Ca) against baculovirus recombinant proteins (minor and nonstructural) derived from bluetongue virus serotype 10. Immunization of mice with recombinant baculovirus insect cell extracts expressing the nonstructural protein NS2 (Bac-NS2) conferred partial protection against infection with vaccinia virus expressing the NS2 protein. This protective immunity was mediated by CD8+ cells. In contrast, no protection was observed when mice were immunized with similarly expressed Bac-NS1 or -NS3 or the virion minor structural proteins (Bac-VP1, -VP4, or -VP6). Furthermore, the in vitro cytotoxicity activity of T cells derived from immunized animals did not correlate to the protective efficacy of baculovirus recombinant proteins. The implications of this work with regard to the design of noninfectious subunit vaccines are discussed.
摘要
针对源自蓝舌病毒10型的杆状病毒重组蛋白（次要和非结构蛋白），在两种品系的小鼠（BALB/c和CBA/Ca）中产生了病毒特异性细胞毒性T淋巴细胞。用表达非结构蛋白NS2的重组杆状病毒昆虫细胞提取物（Bac-NS2）免疫小鼠，可对表达NS2蛋白的痘苗病毒感染提供部分保护。这种保护性免疫由CD8 +细胞介导。相比之下，当用类似表达的Bac-NS1或-NS3或病毒粒子次要结构蛋白（Bac-VP1、-VP4或-VP6）免疫小鼠时，未观察到保护作用。此外，来自免疫动物的T细胞的体外细胞毒性活性与杆状病毒重组蛋白的保护效力无关。讨论了这项工作对非感染性子单位疫苗设计的意义。
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本文引用的文献
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Induction of protective cytotoxic T cells with viral proteins.用病毒蛋白诱导保护性细胞毒性T细胞。
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Characterization of immune responses to baculovirus-expressed equine herpesvirus type 1 glycoproteins D and H in a murine model.在小鼠模型中对杆状病毒表达的马疱疹病毒1型糖蛋白D和H的免疫反应特征分析
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Use of baculovirus expression vectors: development of diagnostic reagents, vaccines and morphological counterparts of bluetongue virus.杆状病毒表达载体的应用：蓝舌病病毒诊断试剂、疫苗及形态类似物的研发
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